LOCUS       sequence-139586-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap syntron-embedded FLP-on N-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS281
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(186..219)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(617..650)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(693..717)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgataagc aatgctttct tataatgcca actttgtaca agaaagctgg gtagctcttc
      121 gcgtgcctcc actaccgccg gtacctccgc tgcccatacc ctgaaaattt aaatcagatt
      181 agttagaagt tcctatactt tctagagaat aggaacttct gatgcgttga agcagtttcc
      241 ctgaattaaa attagaagtt gaaaatacaa tagaaagaaa attggaaggt atataaactc
      301 gcaaaaacga ggaaatggca gattcaataa gatgaataaa tatacaacct ttattgagaa
      361 gagaccattt atatacttgt aagcttctag gaaaatttta gatactaaag agaagcatag
      421 attttaagac aagcagttaa ctaggtgaaa gtaggatgag acagcttaca cattgatcct
      481 agcagaagca caggctgcag ggtgacggtc catcccgctc tcctgggcac aagacatggg
      541 cagcgtgcca tcatcctgct cctccacctc cggcgggaag ccatggctac cctgaaaatt
      601 taaatcagat tagttagaag ttcctatact ttctagagaa taggaacttc gttagtaaaa
      661 tcatgtttaa acttaccgaa cccgaagagc atagcctgct tttttgtaca aagttggcat
      721 tataaaaaag cattgctcat caatttgttg caacgaacag gtcactatca gtcaaata
//
