LOCUS       sequence-139589-         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap FLP-on N-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS282
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    104..137
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    512..545
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(559..658)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
       61 aatgccaact ttgtacaaaa aagcaggcta tgctcttcgg gtggaagttc ctattctcta
      121 gaaagtatag gaacttctag ccatggcttc ccgccggagg tggaggagca ggatgatggc
      181 acgctgccca tgtcttgtgc ccaggagagc gggatggacc gtcaccctgc agcctgtgct
      241 tctgctagga tcaatgtgta agctgtctca tcctactttc acctagttaa ctgcttgtct
      301 taaaatctat gcttctcttt agtatctaaa attttcctag aagcttacaa gtatataaat
      361 ggtctcttct caataaaggt tgtatattta ttcatcttat tgaatctgcc atttcctcgt
      421 ttttgcgagt ttatatacct tccaattttc tttctattgt attttcaact tctaatttta
      481 attcagggaa actgcttcaa cgcatcaatg ggaagttcct attctctaga aagtatagga
      541 acttctacgc gaagagctac ccagctttct tgtacaaagt tggcattata agaaagcatt
      601 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
//
