LOCUS       sequence-139590-         580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap FLP-on N-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS282
FEATURES             Location/Qualifiers
     source          1..580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(126..159)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(534..567)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgataagc aatgctttct tataatgcca actttgtaca agaaagctgg gtagctcttc
      121 gcgtagaagt tcctatactt tctagagaat aggaacttcc cattgatgcg ttgaagcagt
      181 ttccctgaat taaaattaga agttgaaaat acaatagaaa gaaaattgga aggtatataa
      241 actcgcaaaa acgaggaaat ggcagattca ataagatgaa taaatataca acctttattg
      301 agaagagacc atttatatac ttgtaagctt ctaggaaaat tttagatact aaagagaagc
      361 atagatttta agacaagcag ttaactaggt gaaagtagga tgagacagct tacacattga
      421 tcctagcaga agcacaggct gcagggtgac ggtccatccc gctctcctgg gcacaagaca
      481 tgggcagcgt gccatcatcc tgctcctcca cctccggcgg gaagccatgg ctagaagttc
      541 ctatactttc tagagaatag gaacttccac ccgaagagca
//
