LOCUS       sequence-139592-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap egl-13 NLS connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS285
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..43
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     28..44
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(193..209)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(193..209)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(246..266)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(246..264)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(285..301)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(285..301)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(403..422)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(481..500)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
       61 attcctgcag ccctatgctc ttcgggtggg agctctatga gccgtagacg aaaagcgaat
      121 ccgacaaaac tgagtgaaaa cgcgaagaag cttgccaagg aagttgaaaa ttaaacgcga
      181 agagctacgg gggatccact agttctagag cggccgccac cgcggtggag ctccagcttt
      241 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
      301 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
      361 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      421 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
      481 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
      541 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
      601 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
      661 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
      721 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
      781 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
      841 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
      901 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
      961 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     1021 tttttgacga ggggaaatt
//
