LOCUS       sequence-139600-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap STOP codon + let-858 UTR N-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS297
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..43
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     28..44
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(512..528)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(512..528)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(565..585)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(565..583)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(604..620)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(604..620)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(722..741)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(800..819)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
       61 attcctgcag cccggctctt cgggttaaat tttcaaattt taaatactga atatttgttt
      121 tttttcctat tatttattta ttctctttgt gttttttttc ttgctttcta aaaaattaat
      181 tcaatccaaa tctaaacatt tttttttctc tttccgtctc ccaattcgta ttccgctcct
      241 ctcatctgaa cacaatgtgc aagtttattt atcttctcgc tttcatttca ttaggacgtg
      301 gggggaattg gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa taaggacaca
      361 caatatgcaa caacattcaa ttcagaaata tggaggaagg tttaaaagaa aacataaaaa
      421 tatatagagg aggaaggaaa actagtaaaa aataagcaaa gaaattaggc gaacgatgag
      481 aattgtcctc gcttgacggg aagagcgggg ggatccacta gttctagagc ggccgccacc
      541 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
      601 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
      661 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
      721 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
      781 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
      841 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
      901 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
      961 atgcctcttc cgac
//
