LOCUS       sequence-139601-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap STOP codon + let-858 UTR N-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS297
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..71
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     55..71
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(539..555)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(540..556)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(580..599)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(581..599)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(609..626)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(609..625)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(618..640)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      689..775
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgctctaga
       61 actagtggat ccccccgctc ttcccgtcaa gcgaggacaa ttctcatcgt tcgcctaatt
      121 tctttgctta ttttttacta gttttccttc ctcctctata tatttttatg ttttctttta
      181 aaccttcctc catatttctg aattgaatgt tgttgcatat tgtgtgtcct tatttcattt
      241 ccatcatcct tttgtgtgtt tcccccttcc accaattccc cccacgtcct aatgaaatga
      301 aagcgagaag ataaataaac ttgcacattg tgttcagatg agaggagcgg aatacgaatt
      361 gggagacgga aagagaaaaa aaaatgttta gatttggatt gaattaattt tttagaaagc
      421 aagaaaaaaa acacaaagag aataaataaa taataggaaa aaaaacaaat attcagtatt
      481 taaaatttga aaatttaacc cgaagagccg ggctgcagga attcgatatc aagcttatcg
      541 ataccgtcga cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac
      601 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaacatcca tgctagcgtt
      661 aacgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cggaagactg
      721 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
      781 gggagcggat ttgaacgttg tgaagcaacg gcccggaggg tggcggg
//
