LOCUS       sequence-139603-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Simple cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS280
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    11..118
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     27..43
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     28..44
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(78..94)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(78..94)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(131..151)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(131..149)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(170..186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(170..186)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(288..307)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(366..385)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
       61 attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg tggagctcca
      121 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
      181 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
      241 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
      301 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
      361 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
      421 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
      481 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
      541 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
      601 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
      661 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
      721 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
      781 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
      841 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
      901 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
      961 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     1021 acagaaacgg ctttttcaaa aatatggtat tgataatcct ga
//
