LOCUS       sequence-139604-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Simple cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS280
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    29..136
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     53..69
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     53..69
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(103..119)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(104..120)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(144..163)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(145..163)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(173..190)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(173..189)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(182..204)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      253..339
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      431..458
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(617..634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc gctctagaac
       61 tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga
      121 gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
      181 tcgttttaca acgtcgtgac tgggaaaaca tccatgctag cgttaacgcg agagtaggga
      241 actgccaggc atcaaataaa acgaaaggct cagtcggaag actgggcctt tcgttttatc
      301 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
      361 gttgtgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
      421 caaactaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttcct
      481 ggctagcggt acgcgtatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      541 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      601 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      661 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataa
//
