LOCUS       sequence-139605-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway compatible [1-2] Entry Vector encoding 2xNLS-FLP-D5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pWD509
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(113..184)
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
ORIGIN
        1 gctggatggc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gataagcaat gctttcttat aatgccaact ttgtacaaga aagctgggtt tagttctcga
      121 cctccttggc gagcttcttg gcgttctcgg agagcttggt tgggttggcc ttacgacgac
      181 gggagatacg acggttgatg taggaggaga ggtagtcgag gacctcttgg gagatgattc
      241 cgttccaggc tgggtaacgg atggatccct cggcggaacc cttgagttgc tcgatgtgtt
      301 gccactcctc gattgggttg gtctcgtcct tgagggcgat catctccttg gagattgggt
      361 cgtaggcgta gtaacgggag acgagggcga agtagtggtc tgggatggcg gtgatttggt
      421 gggtgtaggt ggtacgggcg acggcggagg cacgcttgtc ggaccagttt ccgacgacgt
      481 tggtgagctc ggtgagtccc ttcatggaga ggaaggaggt catgaggtga cgtccgatgt
      541 gggactttgg tccgttcttg atggcgaaga tggagtatgg ggcgttcttc ttgagggcct
      601 gaaaatttaa atatgtatgg ttagttagta ccgaactgtt taaacttacc ttgttgtagg
      661 aacggacgag gttgtccttg aggagttggt actcttgctt gttggaggag gagtttccgg
      721 tacggttgac acgcttgagg actggctcgg agttacggag gaactcgtcg aggtagacga
      781 gtgggtcgat acgtccacgg gcggagaaga agtagatgtg acgggagacg gaggtcttgg
      841 tctcggtgac gaggcattgg atgatgactc cgaggtactt gttttggacg agcttgaagg
      901 actttgggtc gacgttcttg atgtcggaga aacgtccgca gttgatgaag gtggcgagga
      961 agaggaattg
//
