LOCUS       sequence-139606-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway compatible [1-2] Entry Vector encoding 2xNLS-FLP-D5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pWD509
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             100..120
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagcaggc taaaaaatgc caaagaagaa gcgtaaggtc
      121 ccacaatttg acatcctttg taaaacccca ccaaaggtcc tcgtccgtca attcgtcgag
      181 cgtttcgagc gtccatccgg agagaagatc gccctctgcg ccgccgagct cacctacctc
      241 tgctggatga tcacccacaa cggaaccgcc atcaagcgtg ccaccttcat gtcctacaac
      301 accatcatct ccaactccct ctccttcgac atcgtcaaca agtccctcca attcaagtac
      361 aagacccaaa aggccaccat cctcgaggcc tccctcaaga agctcatccc agcctgggag
      421 ttcaccatca tcccatacta cggacaaaag caccaatccg acatcaccga catcgtctcc
      481 tccctccaac tccaattcga gtcctccgag gaggccgaca agggaaactc ccactccaag
      541 aaggtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcagatgctc
      601 aaggccctcc tctccgaggg agagtccatc tgggagatca ccgagaagat cctcaactcc
      661 ttcgagtaca cctcccgttt caccaagacc aagaccctct accaattcct cttcctcgcc
      721 accttcatca actgcggacg tttctccgac atcaagaacg tcgacccaaa gtccttcaag
      781 ctcgtccaaa acaagtacct cggagtcatc atccaatgcc tcgtcaccga gaccaagacc
      841 tccgtctccc gtcacatcta cttcttctcc gcccgtggac gtatcgaccc actcgtctac
      901 ctcgacgagt tcctccgtaa ctccgagcca gtcctcaagc gtgtcaaccg taccggaaac
      961 tcctcctcca acaagcaaga gtaccaactc ctcaaggaca acctcgtccg ttcctacaac
     1021 aaggtaagtt taaacagttc ggtactaact aaccatacat atttaaa
//
