LOCUS       sequence-139608-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap 3-site Destination vector for N-terminal GFP tagging with 
            embedded Cbr-unc-119 selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS234
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(221..241)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(431..464)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(613..624)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagctggg tctggcgaag
      121 agcccatgga tccgctcttc gaccagtgcc tccactaccg cccgttcctc ctgtgccacc
      181 tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac
      241 catgtggtct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg
      301 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      361 tagttgaacg cttccatctt caatgttgtg tctaattttg aagttctgaa aatttaaatc
      421 agattagtta ataacttcgt atagcataca ttatacgaag ttatctttga gccaatttat
      481 ccaagtcctt gtaaaaagta ttcgaaaatt gttaacggat aaaatgttta ttataatatc
      541 aaaaacaatt tgtcagttga ccactttttg atatatttga cagaaaccgg gatgaattgg
      601 ctcaaagtta gggcgccctt ctattacagg gtttctgata acaaaccggt tattaactcc
      661 caacaaggga tgatttcaat tcatcatgct caaattgacc caaattaagt tacatgacaa
      721 attcatcgcc cttttcaact cttcttggct catcatctgt tattctgttc ctattctgtc
      781 tgcaccctat acccctttgc atactctctc gtcattcctt tcttggatag tgtcttcttc
      841 tccccagctc tgctacttct atgacttgcg cccgctgctt ttccgctcgt tcctctcctc
      901 tctgacgtct tcttctgctt cttttctcct tcctttttcc cttctcaatc ttcctctttt
      961 tcccatttct gtcaatc
//
