LOCUS       sequence-139611-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap 3-site Destination vector for C-terminal GFP tagging with 
            embedded Cbr-unc-119 selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS235
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(185..205)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(395..428)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(577..588)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagctggg tcagtcgaag
      121 agcccatgga tccgctcttc gtcatttgta tagttcatcc atgccatgtg taatcccagc
      181 agctgttaca aactcaagaa ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag
      241 ggcagattgt gtggacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg
      301 agtattttgt tgataatggt ctgctagttg aacgcttcca tcttcaatgt tgtgtctaat
      361 tttgaagttc tgaaaattta aatcagatta gttaataact tcgtatagca tacattatac
      421 gaagttatct ttgagccaat ttatccaagt ccttgtaaaa agtattcgaa aattgttaac
      481 ggataaaatg tttattataa tatcaaaaac aatttgtcag ttgaccactt tttgatatat
      541 ttgacagaaa ccgggatgaa ttggctcaaa gttagggcgc ccttctatta cagggtttct
      601 gataacaaac cggttattaa ctcccaacaa gggatgattt caattcatca tgctcaaatt
      661 gacccaaatt aagttacatg acaaattcat cgcccttttc aactcttctt ggctcatcat
      721 ctgttattct gttcctattc tgtctgcacc ctatacccct ttgcatactc tctcgtcatt
      781 cctttcttgg atagtgtctt cttctcccca gctctgctac ttctatgact tgcgcccgct
      841 gcttttccgc tcgttcctct cctctctgac gtcttcttct gcttcttttc tccttccttt
      901 ttcccttctc aatcttcctc tttttcccat ttctgtcaat cattcgaaga agaagaagaa
      961 gaagaccc
//
