LOCUS       sequence-139616-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Punc-17::2xNLS-FLP-D5 Expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS260
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    436..460
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(464..535)
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
ORIGIN
        1 ttgccaagcg aggacaattc tcatcgttcg cctaatttct ttgcttattt tttactagtt
       61 ttccttcctc ctctatatat ttttatgttt tcttttaaac cttcctccat atttctgaat
      121 tgaatgttgt tgcatattgt gtgtccttat ttcatttcca tcatcctttt gtgtgtttcc
      181 cccttccacc aattcccccc acgtcctaat gaaatgaaag cgagaagata aataaacttg
      241 cacattgtgt tcagatgaga ggagcggaat acgaattggg agacggaaag agaaaaaaaa
      301 atgtttagat ttggattgaa ttaatttttt agaaagcaag aaaaaaaaca caaagagaat
      361 aaataaataa taggaaaaaa aacaaatatt cagtatttaa aatttgaaaa ttcaacgacg
      421 ttggcgtcga tcatccccac tttgtacaag aaagctgggt ttagttctcg acctccttgg
      481 cgagcttctt ggcgttctcg gagagcttgg ttgggttggc cttacgacga cgggagatac
      541 gacggttgat gtaggaggag aggtagtcga ggacctcttg ggagatgatt ccgttccagg
      601 ctgggtaacg gatggatccc tcggcggaac ccttgagttg ctcgatgtgt tgccactcct
      661 cgattgggtt ggtctcgtcc ttgagggcga tcatctcctt ggagattggg tcgtaggcgt
      721 agtaacggga gacgagggcg aagtagtggt ctgggatggc ggtgatttgg tgggtgtagg
      781 tggtacgggc gacggcggag gcacgcttgt cggaccagtt tccgacgacg ttggtgagct
      841 cggtgagtcc cttcatggag aggaaggagg tcatgaggtg acgtccgatg tgggactttg
      901 gtccgttctt gatggcgaag atggagtatg gggcgttctt cttgagggcc tgaaaattta
      961 aatatgtatg gttagttagt accgaactgt ttaaacttac cttgttgtag gaacggacga
     1021 ggttg
//
