LOCUS       sequence-139618-        3200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli cloning vector (AmpR, high copy, blue/white selection, M13 
            IR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Vieira J, Messing J
  TITLE     New pUC-derived cloning vectors with different selectable markers 
            and DNA replication origins.
  JOURNAL   Gene. 1991 Apr;100:189-94.
  PUBMED    1905257
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1991-04"; volume: "100"; pages: "189-94"
COMMENT     Sequence Label: pUC21
FEATURES             Location/Qualifiers
     source          1..3200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    183..199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     188..210
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     207..223
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     207..223
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             219..659
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPLVRGLEIYRCMPWYPGARIRSFCRRVDVIWIRYRRGGRS
                     RTSGSIPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRP
                     SQQLRSLNGEWRLMRYFLLTHLCGISHRIRQSNHSTRPVAAH"
     primer_bind     351..367
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     351..367
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(381..400)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(382..400)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(407..424)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(407..423)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(416..438)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(723..742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     855..876
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1030..1049)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1149..1171
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1209..1227)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1295..1399
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1400..1468,1469..2260)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1618..1637)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2431..3019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2920..2939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3173..3190
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc gcggctcgta tgttgtgtgg
      181 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccactag
      241 tccgaggcct cgagatctat cgatgcatgc catggtaccc gggagctcga attcgaagct
      301 tctgcagacg cgtcgacgtc atatggatcc gatatcgccg tggcggccgc tctagaacta
      361 gtggatcgat ccccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
      421 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      481 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      541 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
      601 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
      661 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
      721 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
      781 tctaaatcgg ggtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
      841 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
      901 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
      961 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     1021 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     1081 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     1141 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     1201 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     1261 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1321 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1381 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1441 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     1501 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     1561 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     1621 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     1681 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     1741 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1801 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1861 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1921 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1981 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     2041 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     2101 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     2161 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     2221 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     2281 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     2341 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2401 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2461 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2521 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2581 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2641 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2701 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2761 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2821 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2881 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2941 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3001 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3061 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3121 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3181 agtgagcgag gaagcggaag
//
