LOCUS       sequence-139626-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRuby2_PUFa in pmax-DEST expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1448_pmax-mRuby2_PUFa
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..100
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(104..112,113..127)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 agcaggctcc gaattcaccg gtgccgccac catggtgcgg ggttctcatc atcatcatca
       61 tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga
      121 cgataaggat ccgatggtgt ctaagggcga agagctgatc aaggaaaata tgcgtatgaa
      181 ggtggtcatg gaaggttcgg tcaacggcca ccaattcaaa tgcacaggtg aaggagaagg
      241 caatccgtac atgggaactc aaaccatgag gatcaaagtc atcgagggag gacccctgcc
      301 atttgccttt gacattcttg ccacgtcgtt catgtatggc agccgtactt ttatcaagta
      361 cccgaaaggc attcctgatt tctttaaaca gtcctttcct gagggtttta cttgggaaag
      421 agttacgaga tacgaagatg gtggagtcgt caccgtcatg caggacacca gccttgagga
      481 tggctgtctc gtttaccacg tccaagtcag aggggtaaac tttccctcca atggtcccgt
      541 gatgcagaag aagaccaagg gttgggagcc taatacagag atgatgtatc cagcagatgg
      601 tggtctgagg ggatacactc atatggcact gaaagttgat ggtggtggcc atctgtcttg
      661 ctctttcgta acaacttaca ggtcaaaaaa gaccgtcggg aacatcaaga tgcccggtat
      721 ccatgccgtt gatcaccgcc tggaaaggtt agaggaaagt gacaatgaaa tgttcgtagt
      781 acaacgcgaa cacgcagttg ccaagttcgc cgggcttggt ggtgggatgg
//
