LOCUS       sequence-139627-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRuby2_PUFa in pmax-DEST expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1448_pmax-mRuby2_PUFa
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..52
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagcggccgc cactgtgctg gatatcaacc actttgtaca agaaagctgg gtcgaattct
       61 taattaatca ccctaagtca acaccgttct tcatgtagta cttctccagc ttggccagaa
      121 tgtgcttgcc ataggtgtac ttacgaagag ttgcgatgtg gggccggatc ttatgcatga
      181 cgatcttccg ctggcctggc tccgccacgt caatcatctt ctggaccacg tagttggcat
      241 actggtcctt catcatggtg tataaggcac tgtggggacc gtcgttcatg gtgcacacct
      301 catcgatgag cacagcgcgc tccgtacgtg aggcgtgagt aacacacttc tccacaacat
      361 tgcttgcaaa tttgtgctga ctcaatacaa gtacattgcc tcggatttct gctacaattt
      421 tgcttttatc ctcaggacga ccgtgctcca gtacatgttg gattacataa tttccatatt
      481 gatcctgtac cagctgctct gtgtgctggt gaagctcctc taaaataggg agtgtctggt
      541 cagggagaca gtgctccagg attctctgaa tcactcggca gccataagga tgtgtggata
      601 aggcaaatac ctgtccctta aacgcatcga tgataaattg caaagactgg ggctgtacac
      661 attcaatgca tttctgaacc acgtgattgc cattctgatc tttcacacac ttcaagacat
      721 ggccatctag ttcccgaacc atctcattct gctggtctga aggaataaac tcaagagctt
      781 tctcgataac acggctgcca tacatctgta gtgccaatga caggacgtgg cctcgaatcc
      841 gttctgccaa agccagcttc tgttcaagac tg
//
