LOCUS       sequence-139628-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KRAB_PUFa in pmax-DEST expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1449_pmax-KRAB_PUFa
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..60
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             100..294
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             325..345
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcaggctccg aattcaccgg tgccgccacc atggggagcc ccaagaagaa gagaaaggtg
       61 gaggccagca tggacgcgaa atcacttacg gcatggtcga gaacactggt tacgttcaag
      121 gacgtgtttg tggactttac acgtgaggag tggaaattgc tggatactgc gcaacaaatt
      181 gtgtatcgaa atgtcatgct tgagaattac aagaacctcg tcagtctcgg ataccagttg
      241 acgaaaccgg atgtgatcct taggctcgaa aagggggaag aaccttggct ggtatctaga
      301 ggcagtatcg ttgggatcct cccccccaag aaaaagagga aggtatctag aggccgcagc
      361 cgccttttgg aagattttcg aaacaaccgg taccccaatt tacaactgcg ggagattgct
      421 ggacatataa tggaattttc ccaagaccag catgggtcca gattcattca gctgaaactg
      481 gagcgtgcca caccagctga gcgccagctt gtcttcaatg aaatcctcca ggctgcctac
      541 caactcatgg tggatgtgtt tggtaattac gtcattcaga agttctttga atttggcagt
      601 cttgaacaga agctggcttt ggcagaacgg attcgaggcc acgtcctgtc attggcacta
      661 cagatgtatg gcagccgtgt tatcgagaaa gctcttgagt ttattccttc agaccagcag
      721 aatgagatgg ttcgggaact agatggccat gtcttgaagt gtgtgaaaga tcagaatggc
      781 aatcacgtgg ttcagaaatg cattgaatgt gtacagcccc agtctttgca atttatcatc
      841 gatgcgttta agggacaggt atttgcctta tccacacatc cttatggctg ccgagtgatt
      901 c
//
