LOCUS       sequence-139629-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KRAB_PUFa in pmax-DEST expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1449_pmax-KRAB_PUFa
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    35..59
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tagactcgag cggccgccac tgtgctggat atcaaccact ttgtacaaga aagctgggtc
       61 gaattcttaa ttaatcaccc taagtcaaca ccgttcttca tgtagtactt ctccagcttg
      121 gccagaatgt gcttgccata ggtgtactta cgaagagttg cgatgtgggg ccggatctta
      181 tgcatgacga tcttccgctg gcctggctcc gccacgtcaa tcatcttctg gaccacgtag
      241 ttggcatact ggtccttcat catggtgtat aaggcactgt ggggaccgtc gttcatggtg
      301 cacacctcat cgatgagcac agcgcgctcc gtacgtgagg cgtgagtaac acacttctcc
      361 acaacattgc ttgcaaattt gtgctgactc aatacaagta cattgcctcg gatttctgct
      421 acaattttgc ttttatcctc aggacgaccg tgctccagta catgttggat tacataattt
      481 ccatattgat cctgtaccag ctgctctgtg tgctggtgaa gctcctctaa aatagggagt
      541 gtctggtcag ggagacagtg ctccaggatt ctctgaatca ctcggcagcc ataaggatgt
      601 gtggataagg caaatacctg tcccttaaac gcatcgatga taaattgcaa agactggggc
      661 tgtacacatt caatgcattt ctgaaccacg tgattgccat tctgatcttt cacacacttc
      721 aagacatggc catctagttc ccgaaccatc tcattctgct ggtctgaagg aataaactca
      781 agagctttct cgataacacg gctgccatac atctgtagtg ccaatgacag gacgtggcct
      841 cgaatccgtt ctgccaaag
//
