LOCUS       sequence-13963-e        6656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Willott E, Balda MS, Fanning AS, Jameson B, Van Itallie C, Anderson 
            JM
  TITLE     The tight junction protein ZO-1 is homologous to the Drosophila 
            discs-large tumor suppressor protein of septate junctions.
  JOURNAL   Proc Natl Acad Sci U S A. 1993 Aug 15. 90(16):7834-8.
  PUBMED    8395056
REFERENCE   2  (bases 1 to 6656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1993 Aug 15. 90(16):7834-8."
COMMENT     Sequence Label: pSK ZO1
FEATURES             Location/Qualifiers
     source          1..6656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1857..1865
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gaattccctc cccctgggcc gggcccttcc gtccgccccc gcccgtgccc cgctcgctct
       61 cgggagatgt ttatttgggc tgtggcgtga ggagcgggcg ggccagcgcc gcggagtttc
      121 gggtccgagg agcctcgcgc ggcgctggag agagacaaga tgtccgccag agctgcggcc
      181 gccaagagca cagcaatgga ggaaacagct atatgggaac aacatacagt gacgcttcac
      241 agggctcctg gatttggatt tggaattgca atatctggtg gacgagataa tcctcatttt
      301 cagagtgggg aaacgtcaat agtgatttca gatgtgctga aaggaggacc agctgaagga
      361 cagctacagg aaaatgaccg agttgcaatg gttaacggag tttcaatgga taatgttgaa
      421 catgcttttg ctgttcagca actaaggaaa agtgggaaaa atgcaaaaat tacaattaga
      481 aggaagaaga aagttcaaat accagtaagt cgtcctgatc ctgaaccagt atctgataat
      541 gaagaagata gttatgatga ggaaatacat gatccaagaa gtggccggag tggtgtggtt
      601 aacagaagga gtgagaagat ttggccgagg gatagaagtg caagtagaga gaggagcttg
      661 tccccgcggt cagacaggcg gtcagtggct tccagccagc ctgctaaacc tactaaagtc
      721 acactggtga aatcccggaa aaatgaagaa tatggtcttc gattggcaag ccatatattt
      781 gttaaggaaa tttcacaaga tagtttggca gcaagagatg gcaatattca agaaggtgat
      841 gttgtattga agataaatgg tactgtgaca gaaaatatgt cattgacaga tgcaaagaca
      901 ttgatagaaa ggtctaaagg caaattaaaa atggtagttc aaagagatga acgggctacg
      961 ctattgaatg tccctgatct ttctgacagc atccactctg ctaatgcctc tgagagagac
     1021 gacatttcag aaattcagtc actggcatca gatcattctg gtcgatcaca cgataggcct
     1081 ccccgccgca gccggtcacg atctcctgac cagcggtcag agccttctga tcattccagg
     1141 cactcgccgc agcagccaag caatggcagt ctccggagta gagatgaaga gagaatttct
     1201 aaacctgggg ctgtctcaac tcctgtaaag catgctgatg atcacacacc taaaacagtg
     1261 gaagaagtta cagttgaaag aaatgagaaa caaacacctt ctcttccaga accaaagcct
     1321 gtgtatgccc aagttgggca accagatgtg gatttacctg tcagtccatc tgatggtgtc
     1381 ctacctaatt caactcatga agatgggatt cttcggccca gcatgaaatt ggtaaaattc
     1441 agaaaaggag atagtgtggg tttgcggctg gctggtggaa atgatgttgg aatatttgta
     1501 gctggcgttc tagaagatag ccctgcagcc aaggaaggct tagaggaagg tgatcaaatt
     1561 ctcagggtaa acaacgtaga ttttacaaat atcataagag aagaagccgt ccttttcctg
     1621 cttgacctcc ctaaaggaga agaagtgacc atattggctc agaagaagaa ggatgtttat
     1681 cgtcgcattg tagaatcaga tgtaggagat tctttctata ttagaaccca ttttgaatat
     1741 gaaaaggaat ctccctatgg acttagtttt aacaaaggag aggtgttccg tgctgtggat
     1801 accttgtaca atggaaaact gggctcttgg cttgctattc gaattggtaa aaatcataag
     1861 gaggtagaac gaggcatcat ccctaataag aacagagctg agcagctagc cagtgtacag
     1921 tatacacttc caaaaacagc aggcggagac cgtgctgact tctggagatt cagaggtctt
     1981 cgcagctcca agagaaatct tcgaaaaagc agagaggatt tgtccgctca gcctgttcaa
     2041 acaaagtttc cagcttatga aagagtggtt cttcgagaag ctggatttct gaggcctgta
     2101 accatttttg gaccaatagc tgatgttgcc agagaaaagc tggcaagaga agaaccagat
     2161 atttatcaaa ttgcaaagag tgaaccacga gacgctggaa ctgaccaacg tagctctggc
     2221 tatattcgcc tgcatacaat aaagcaaatc atagatcaag acaaacatgc tttattagat
     2281 gtaacaccaa atgcagttga tcgtcttaac tatgcccagt ggtatccaat tgttgtattt
     2341 cttaaccctg attctaagca aggagtaaaa acaatgagaa tgaggttatg tccagaatct
     2401 cggaaaagtg ccaggaagtt atacgagcga tctcataaac ttgctaaaaa taatcaccat
     2461 ctttttacaa ctacaattaa cttaaattca atgaatgatg gttggtatgg tgcgctgaaa
     2521 gaagcagttc aacaacagca aaaccagctg gtatgggttt ccgagggaaa ggcggatggt
     2581 gctacaagtg atgaccttga tttgcatgat gatcgtctgt cctacctgtc agctccaggt
     2641 agtgaatact caatgtatag cacggacagt agacacactt ctgactatga agacacagac
     2701 acagaaggcg gggcctacac tgatcaagaa ctagatgaaa ctcttaatga tgaggttggg
     2761 actccaccgg agtctgccat tacacggtcc tctgagcctg taagagagga ctcctctgga
     2821 atgcatcatg aaaaccaaac atatcctcct tactcaccac aagcgcagcc acaaccaatt
     2881 catagaatag actcccctgg atttaagcca gcctctcaac agaaagcaga agcttcatct
     2941 ccagtccctt acctttcgcc tgaaacaaac ccagcatcat caacctctgc tgttaatcat
     3001 aatgtaaatt taactaatgt cagactggag gagcccaccc cagctccttc cacctcttac
     3061 tcaccacaag ctgattcttt aagaacacca agtactgagg cagctcacat aatgctaaga
     3121 gatcaagaac catcattgtc gtcgcatgta gatccaacaa aggtgtatag aaaggatcca
     3181 tatcccgagg aaatgatgag gcagaaccat gttttgaaac agccagccgt tagtcaccca
     3241 gggcacaggc cagacaaaga gcctaatctg acctatgaac cccaactccc atacgtagag
     3301 aaacaagcca gcagagacct cgagcagccc acatacagat acgagtcctc aagctatacg
     3361 gaccagtttt ctcgaaacta tgaacatcgt ctgcgatacg aagatcgcgt ccccatgtat
     3421 gaagaacagt ggtcatatta tgatgacaaa cagccctacc catctcggcc accttttgat
     3481 aatcagcact ctcaagacct tgactccaga cagcatcccg aagagtcctc agaacgaggg
     3541 tactttccac gttttgaaga gccagcccct ctgtcttacg acagcagacc acgttacgaa
     3601 caggcaccta gagcatccgc cctgcggcac gaagagcagc cagctcctgg gtatgacaca
     3661 catggtagac tcagaccgga agcccagccc cacccttcag cagggcccaa gcctgcagag
     3721 tccaagcagt attttgagca atattcacgc agttacgagc aagtaccacc ccaaggattt
     3781 acctctagag caggtcattt tgagcctctc catggtgctg cagctgtccc tccgctgata
     3841 ccttcatctc agcataagcc agaagctctg ccttcaaaca ccaaaccact gcctccaccc
     3901 ccaactcaaa ccgaagaaga ggaagatcca gcaatgaagc cacagtctgt actcaccaga
     3961 gttaagatgt ttgaaaacaa aagatctgca tccttagaga ccaagaagga tgtaaatgac
     4021 actggcagtt ttaagcctcc agaagtagca tctaaacctt caggtgctcc catcattggt
     4081 cccaaaccca cttctcagaa tcaattcagt gaacatgaca aaactctgta caggatccca
     4141 gaacctcaaa aacctcaact gaagccacct gaagatattg ttcggtccaa tcattatgac
     4201 cctgaagaag atgaagaata ttatcgaaaa cagctgtcat actttgaccg aagaagtttt
     4261 gagaataagc ctcctgcaca cattgctgcc agccatctct ccgagcctgc aaagccagct
     4321 cattctcaga atcaatcaaa tttttctagt tattcttcaa agggaaagcc tcctgaagct
     4381 gatggtgtgg atagatcatt tggcgagaaa cgctatgaac ccatccaggc cactccccct
     4441 cctcctccat tgccctcgca gtatgcccag ccatctcagc ctgtcaccag cgcgtctctc
     4501 cacatacatt ctaagggagc acatggtgaa ggtaattcag tgtcattgga ttttcagaat
     4561 tccttagtgt ccaaaccaga cccacctcca tctcagaata agccagcaac tttcagacca
     4621 ccaaaccgag aagatactgc tcaggcagct ttctatcccc agaaaagttt tccagataaa
     4681 gccccagtta atggaactga acagactcag aaaacagtca ctccagcata caatcgattc
     4741 acaccaaaac catatacaag ttctgcccga ccatttgaac gcaagtttga aagtcctaaa
     4801 ttcaatcaca atcttctgcc aagtgaaact gcacataaac ctgacttgtc ttcaaaaact
     4861 cccacttctc caaaaactct tgtgaaatcg cacagtttgg cacagcctcc tgagtttgac
     4921 agtggagttg aaactttctc tatccatgca gagaagccta aatatcaaat aaataatatc
     4981 agcacagtgc ctaaagctat tcctgtgagt ccttcagctg tggaagagga tgaagatgaa
     5041 gatggtcata ctgtggtggc cacagcccga ggcatattta acagcaatgg gggcgtgctg
     5101 agttccatag aaactggtgt tagtataatt atccctcaag gagccattcc cgaaggagtt
     5161 gagcaggaaa tctatttcaa ggtctgccgg gacaacagca tccttccacc tttagataaa
     5221 gagaaaggtg aaacactgct gagtcctttg gtgatgtgtg gtccccatgg cctcaagttc
     5281 ctgaagcctg tggagctgcg cttaccacac tgtgatccta aaacctggca aaacaagtgt
     5341 cttcccggag atccaaatta tctcgttgga gcaaactgtg tttctgtcct tattgaccac
     5401 ttttaactct tgaaatatag gaacttaaat aatgtgaaac tggattaaac ttaatctaaa
     5461 tggaaccact ctatcaagta ttataccttt tttagagttg atactacagt ttgttagtat
     5521 gaggcatttg tttgaactga taaagatgag tgagcatgcc cctgaaccat ggtcggaaaa
     5581 catgctacac actgcatgtt tgtgattgac gggactgttg gtattggcta gaggttcaaa
     5641 gatattttgc tttgtgattt ttgtaatttt tttatcgtca ctgcttaact tcacatattg
     5701 atttccgtta aaataccagc cagtaaatgg gggtgcattt gaggtctgtt ctttccaaag
     5761 tacactgttt caaactttac tatggccctg gcctagcata cgtacacatt ttattttatt
     5821 atgcatgaag taatatgcac acatttttta aatgcacctg gaatatataa ccagtgttgt
     5881 ggatttaaca gaaatgtaca gcaaggagat ttacaactgg gggagggtga agtgaagaca
     5941 atgacttact gtacatgaaa acacattttt cttagggaag gatacaaaag catgtgagac
     6001 tggttccatg gcctcttcag atctctaact tcaccatatt accacagaca tactaaccag
     6061 cagaaatgcc ttaccctcat gttcttaatt cttagctcat tctccttgtg ttactaagtt
     6121 tttatggctt ttgtgcatta tctagatact gtatcatgac aaagactgag tacgttgtgc
     6181 atttggtggt ttcagaaatg tgttatcacc cagaagaaaa tagtggtgtg atttggggat
     6241 atttttttct tttcttttct tttctttttt tttttttttt gacaaggggc agtggtggtt
     6301 ttctgttctt tctggctatg catttgaaaa ttttgatgtt ttaaggatgc ttgtacataa
     6361 tgcgtgcata ccacttttgt tcttggtttg taaattaact tttataaact ttaccttttt
     6421 tatacataaa caagaccacg tttctaaagg ctacctttgt attctctcct gtacctcttg
     6481 agccttgaac tttgacctct gcagcaataa agcagcgttt ctatgacaca tgcaaggtca
     6541 ttttttttaa gaaaaaggat gcacagagtt gttacatttt taagtgctgc atttaaaaga
     6601 tacagttact cagaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag gaattc
//
