LOCUS       sequence-139631-       15735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP for monosynaptic circuit mapping at the synapse.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15735)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 15735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS N2c(deltaG)-synaptophysin-GFP
FEATURES             Location/Qualifiers
     source          1..15735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4264..4273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4270..4272,4273..4275,4276..4989)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4270..4272,4273..4275,4276..4986)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4315..4336)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4576..4595)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4923..4944
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7164..7175
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(8967..8978)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12175..12193)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12189..12236
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12264..12280)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12264..12280)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12277..12299)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12288..12304
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12312..12318,12319..12336,12337..12342))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12357..12378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12495..12512)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12666..13254)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12746..12765)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13425..14216,14217..14285))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14048..14067
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14286..14390)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14417..14872
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14504..14523)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14714..14735
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14998..15020
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15012..15029
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15013..15029
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15099..15402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15403..15606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     15557..15577
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     15651..15670
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15651..15669
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggac gtggtgaatc agctggtggc tgggggtcag
     3361 ttccgggtgg tcaaggagcc ccttggcttc gtgaaggtgc tgcagtgggt ctttgccatc
     3421 ttcgcctttg ctacgtgcgg cagctacacc ggagagcttc ggctgagcgt ggagtgtgcc
     3481 aacaagacgg agagtgccct caacatcgaa gtcgaatttg agtacccatt caggctgcac
     3541 caagtgtact ttgatgcacc ctcctgcgtt aaagggggca ctaccaagat cttcctagtt
     3601 ggtgactact cctcctcggc tgaattcttt gtcaccgtgg ctgtgtttgc cttcctctac
     3661 tccatggggg ccctggccac ctacatcttc ctgcagaaca agtaccgaga gaacaacaaa
     3721 gggccaatga tggacttcct ggccacagca gtgttcgctt tcatgtggct agttagctca
     3781 tccgcctggg ccaaaggcct gtccgatgtg aagatggcca ctgacccaga gaacattatc
     3841 aaggagatgc ctatgtgccg ccagacagga aacacatgca aggaactgag ggaccctgtg
     3901 acttcaggac tcaacacctc ggtggtgttt ggcttcctga acctggtgct ctgggttggc
     3961 aacctatggt tcgtgttcaa ggagacaggc tgggccgccc cattcatgcg cgcacctcca
     4021 ggcgccccag aaaagcaacc agctcctggc gatgcctacg gcgatgcggg ctatgggcag
     4081 ggccccggag gctatgggcc ccaggactcc tacgggcctc agggtggtta tcaacccgat
     4141 tacgggcagc cagccagcgg cggtggcggt ggctacgggc ctcagggcga ctatgggcag
     4201 caaggctacg gccaacaggg tgcgcccacc tccttctcca atcagatgtc ggatccaccg
     4261 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     4321 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     4381 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     4441 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     4501 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4561 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     4621 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4681 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     4741 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     4801 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4861 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     4921 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     4981 tacaagtaag ctagcggcgc ctagccggtc atccttttga cgattccagt cccgaggata
     5041 acctcctctc gggattgggg ggaatctttg gatccagtag tcctccttga actccgtcca
     5101 acagggcaga ttcaagagtc ataagacttt cattaatcat ttcagttgat cagacatggt
     5161 cgtgtagatt ctcttaatac gggagatctt ctagcagttt cagtgaccaa cggtgctttc
     5221 attctccagg aactgatacc aaaggttgtg gacaggccaa ggggtacttc ggatgactct
     5281 gtgcttgggc acagaaagag gtcgtagtgt gccccccgat agcggactcg acatgaatca
     5341 actaagaaag gcaatctgcc tcccatgatg gacataagca atagttcaca accatcttgc
     5401 atctcagtga agtgtacata actattgagg gctgggtcat ctaagcattt cagtcgagaa
     5461 aaaaactgta gaccaaaaga acaactagca acacttctca tccagagacc catatcaaga
     5521 tgctagatcc gggagaggtt tatgatgacc ctattgatcc aattgagtca gaggctgaac
     5581 ccagaggaac ccccactgtc cccaacatct tgaggaactc cgactacaat ctcaattctc
     5641 ctttgataga ggatcctgcc aaactaatgt tagaatggtt gaagacaggg aacagacctt
     5701 atcggatgac tttgacagac aattgctcca ggtcttacaa agttttgaaa gattatttca
     5761 agaaagtaga tttgggttct ctcaaagtgg gcggaactgc tgcacagtca atggtttctc
     5821 tctggttgtg tggtgcccac tctgaatcaa acaggagccg gagatgtata accgacttgg
     5881 cccatttcta ttccaagtca tcccccatag agaagctatt gaattgtacg ctaggaaaca
     5941 gaggcctgag aatcccacca gagggggtgt taaattgcct cgagagggtc aattatgaca
     6001 aggcatttgg gaggtatctg gccaacacgt attcctctta cttgtttttc catgtaatca
     6061 ccttatacat gaatgcctta gactgggaag aggaaaagac catcctggca ttatggaaag
     6121 atataacctc agtggatacc gagaaggact tggtcaaatt caaagatcaa atatggggac
     6181 tgttgattgt gacaaaggac tttgtttact ctcagagttc aaactgtctt tttgacagaa
     6241 actacacact gatgctaaag gatcttttct tgtctcgatt caactcctta atgattctgc
     6301 tttctccccc tgagccccga tactcagatg acttaatatc tcagctgtgc cagctataca
     6361 tcgctgggga tcaagtcttg tccatgtgtg ggaactccgg ctatgaagtc atcaaaatat
     6421 tggagccata tgtcgtgaac agtttggtcc agagggcaga gaagtttagg cctctcatcc
     6481 accccttggg agactttcct atgttcataa aagacaaggt gaatcaactt gaagggactt
     6541 ttggtcccag tgcaaagagg ttttttaggg ttttagatca attcgacaac atacatgact
     6601 tagtatttgt gtatggctgt tacagacatt gggggcaccc ctatatagat tatcggaagg
     6661 gtctgtcgaa actatatgat caagttcaca ttaagaaagt aatagataag tcctaccagg
     6721 agtgtttagc aagtgacttg gccagaagga tcctcagatg gggatttgac aagtactcca
     6781 aatggtatct agattcgaga ttccttgccc gagaccaccc cttgactcct tatgtcaaga
     6841 cccaaacatg gccacccaaa catatagtag acttggtggg ggacacatgg cataagctcc
     6901 cgatcacgca gatctttgaa attcctgaat caatggaccc gtcagagata ctggatgaca
     6961 aatcacattc tttcaccaga acaaggttag cttcttggct gtcagagaac cgaggggggc
     7021 ctgttcctag cgagaaggtc attatcacgg ccctgtctaa gccacctgtc aatccccgag
     7081 agtttttgaa atctatagac ctcggaggat tgccagatga ggatttgata attggcctca
     7141 aaccaaagga acgggagttg aagatcgagg gccgattctt tgctctaatg tcatggaatc
     7201 taagattata ttttgtcatc accgaaaagc tcttggccaa ctacattttg ccactttttg
     7261 acgcactgac tatgacagac aacctgaaca aggtgttcaa aaagttgatc gacagggtca
     7321 ccgggcaagg gcttttggac tattctaggg tcacatacgc atttcacctg gactatgaga
     7381 aatggaacaa tcatcaaaga ttggagtcaa cagaggatgt attctctgtc ctagatcagg
     7441 tgtttggatt gaagagggtg ttttctagaa cacacgagtt ttttcagaag tcctggatct
     7501 attattcaga cagatcagac ctcattgggt tatgggagga ccaaatatat tgcttggata
     7561 tgtctaacgg cccaacctgc tggaatggcc aagatggcgg gctagagggc ttgcggcaga
     7621 agggctggag tctagtcagt ttattaatga tagatagaga atctcaaacc aggaacacaa
     7681 gaaccaagat actagctcaa ggagacaacc aggttctgtg tcctacatac atgttgtcac
     7741 cgggattgtc tcaagagggg cttctctatg agttagagag catatcaagg aatgcactct
     7801 caatataccg agctatcgag gaaggagcat ctaagctggg gctgatcatc aagaaggaag
     7861 agaccatgtg tagttatgac tttctcatat atgggaagac ccccttattt cgaggcaaca
     7921 tattggtgcc tgaatccaaa agatgggccc gagtctcttg catctctaac gaccaaatag
     7981 tcaacctcgc caatataatg tcgacagtat ccaccaatgc gctgacagtg gcacaacact
     8041 ctcaatctct gatcaaacct atgagggatt ttctgctcat gtcagtacag gcagttttcc
     8101 actacctgtt gtttagccca atcctaaaag gcagagttta taagattctg agtgctgaag
     8161 gggagagctt tctcctagcc atgtcgcgga taatctacct agatccttct ttgggagggg
     8221 tgtctggaat gtctctcggg aggttccata tacgtcagtt ctcagaccct gtctctgaag
     8281 ggttgtcatt ctggagagag atctggttag gctctcatga gtcctggatt cacgcgttgt
     8341 gtcaggaggc cgggaacccc gatcttggag agagaacact cgagagcttc actcgccttt
     8401 tagaagatcc tactacctta aatatcaaag gaggggccag ccctaccatt ctactcaagg
     8461 atgctatcag aaaggctctg tacgacgagg tggacaaggt ggaaaattca gagtttcgag
     8521 aggcaatcct gctgtccaag acccatagag ataactttat actcttttta aaatctgttg
     8581 agcctctgtt ccctcgattt ctcagtgagc tcttcagttc gtctttcttg ggaataccgg
     8641 agtcaatcat tggactgata caaaactccc ggacaataag aaggcagttt agaaagagtc
     8701 tctcaagaac tttagaagag tccttctaca actcagagat ccacgggatt aatcggataa
     8761 cccagacacc tcaaagggtc ggaagggtgt ggccttgctc ttcagagagg gcggatctac
     8821 ttagggagat ctcttggggg aggaaagtgg taggcacgac agttcctcac ccttccgaga
     8881 tgttggagtt gtttcccaaa tcctccattt cctgcacttg tggagcaaca gggggaggca
     8941 atcctagagt ctctgtatca gtactcccgt ccttcgatca gtcatttttc tcacggggcc
     9001 ccctaaaggg atacttgggc tcgtccacct ctatgtcaac ccagctattc catgcatggg
     9061 aaaaagtcac taatgttcat gtggtgaaaa gggctctatc gttaaaagaa tctataaact
     9121 ggttcatcaa taggaattcc aatttggctc aaactctaat tggaaacatc atgtctctga
     9181 caggccctga cttccctcta gaagaggccc ctgttttcaa acggacaggg tcagccttgc
     9241 ataggttcaa gtctgccaga tacagcgaag gagggtattc ttctgtttgc cctaaccttc
     9301 tctcccatat ctctgttagt acagacacta tgtctgattt gacccaaaac gggaagaact
     9361 atgatttcat gtttcagcca ttgatgcttt atgcgcaaac atggacatcg gaactggtac
     9421 agagggatac aagacttaga gactccacgt ttcactggca ccttcggtgc aacagatgtg
     9481 tgaggcccat tgatgatata acactggaaa cttctcagat cttcgagttc ccggatgtgt
     9541 caaaaaggat atccaggatg gtttctggag ctgtccctca atttcagaag cttcctgata
     9601 tccgtctaag accaggtgat tttgaatccc taagtggtag agaaaagtct cgccatatag
     9661 ggtcagctca ggggctctta tactcaatct tagtagcaat tcacgactca ggatacaatg
     9721 atgggaccat cttccctgtc aacatatacg gcaaagtttc ccccagagac tatttgagag
     9781 ggcttgcaag agggatcttg atagggtcct cgatttgctt cttgacacga atgacaaata
     9841 ttaacattaa cagacctctt gaattgatct caggggtaat ttcctatatt ctcctgaggc
     9901 tggataatca tccctctctg tatataatgc tcagagaacc gtctcttaga ggagaaatat
     9961 tctctatccc tcagaaaatc cccgccgctt acccaaccac tatgagagaa ggcaacagat
    10021 cgattttgtg ttacctccaa cacgtgctac gctatgagcg agaggcaatc acggcgtccc
    10081 cggagaatga ctggctgtgg atcttctcag acttcagaag tgtgaaaatg acgtacttga
    10141 ccctcattac ttaccagtct cacctcctac tccagagggt tgagagaaac ttgtctaaga
    10201 gtatgagagc tactctgcga caaatgggtt ccttaatgag gcaagtgctg ggtgggcacg
    10261 gagaagatac cttggagtca gacgatgaca ttcaacgatt actaaaagac tctttgcgaa
    10321 agacaaggtg ggtggatcaa gaggtgcgcc atgcagctag aaccatgaat ggagattaca
    10381 gccccgacaa gaaagtatcc cgtaaggtag gatgttcaga atgggtctgc tctgctcaac
    10441 agattgccgt ctccacctca gccaacccgg cccctgtctc agagcttgac attagggccc
    10501 tctctaagcg gtttcaaaac cccttgatct cgggcctgag agtggttcag tgggcaaccg
    10561 gtgcccatta taagcttaag cctattctag atgatctaaa tgttttccca tctctctgtc
    10621 ttgtaattgg agacgggtca gggggaatat caagggcagt tctcaacatg tttccagatt
    10681 ctaagcttgt gttcaacagc ctattggagg tgaatgatct gatggcttcc ggaacacatc
    10741 cactgcctcc ttcagcaatc atgagtggag gagatgacat catctccaga gtgatagact
    10801 ttgactcaat atgggagaaa ccgtccgacc tgaggaactc ggccacctgg agatacttcc
    10861 agtcggttca aaaacaggtc aacatgtcgt atgacctcat tatttgtgat gcagaagtta
    10921 ctgatattgc atctatcaac cggataactc tgttgatgtc tgatttcgca ttgtctatag
    10981 atggaccact ttatctggtc ttcaaaactt acgggactat gctagtaaac ccggactata
    11041 aagctattca acatctgtca agagcgttcc cttcggtcac agggtttgta acccaagtaa
    11101 cttcatcctt ttcttctgag ctatacctcc ggttctctaa acgaggaaag tttttcaggg
    11161 acgccgagta cttgacctct tccacccttc gagagatgag ccttgtgttg ttcaattgca
    11221 gcagccccaa aagtgagatg cagagagctc gttccttaaa ctatcaagac ctggtaaggg
    11281 gatttcctga agagatcata tcaaatcctt acaacgaaat gatcataact ctgattgaca
    11341 atgatgtaga gtccttccta gtccacaaga tggtggatga tcttgagcta cagaggggaa
    11401 ctctgtctaa agtggctatc attatatcca tcatgatcgt tttttccaat agagtcttca
    11461 acatttccaa acctttgact gaccccttgt tctatccacc gtctgatcct aaaatcctga
    11521 ggcacttcaa catatgttgc agtactatga tgtatctatc taccgcttta ggcgacgtcc
    11581 ctaacttcgc aagacttcat gacctgtata acagacctat aacttgttac ttcagaaaac
    11641 aagttattcg agggaatatt tatctatctt ggagttggtc cgatgatacc ccagtgttca
    11701 agagagtagc ctgtaattct agcttgagtc tgtcatctca ctggatcagg ttgatctaca
    11761 agatagtgaa gactaccaga ctcattggca gcataaaaga cctatcagga gaggtagaac
    11821 gacatcttca tgggtataac agatggatca ccctcgagga tatccgatct agatcatccc
    11881 tactagatta cagttgtttg taagccggat attaccgaaa gcctgtgcat gctaaaattc
    11941 ttgtatgatg catcttgaaa aaaacaagat cttgaatccg gacctctggt tgtttgattg
    12001 ttttttccat ctttattgtt tttttgttaa gcgtgggtcg gcatggcatc tccacctcct
    12061 cgcggtccga cctgggcatc cgaaggagga cgcacgtcca ctcggatggc taagggaggg
    12121 gcggggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
    12181 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
    12241 aagtcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    12301 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    12361 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    12421 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    12481 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    12541 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    12601 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    12661 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    12721 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    12781 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    12841 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    12901 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    12961 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    13021 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    13081 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
    13141 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    13201 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    13261 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    13321 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    13381 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    13441 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    13501 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    13561 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    13621 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    13681 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    13741 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    13801 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    13861 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    13921 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    13981 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    14041 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    14101 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    14161 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    14221 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    14281 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    14341 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    14401 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    14461 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    14521 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    14581 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    14641 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    14701 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    14761 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    14821 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc
    14881 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
    14941 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
    15001 agtcacgacg ttgtaaaacg acggccagtg agcgcgccta gttattaata gtaatcaatt
    15061 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
    15121 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
    15181 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
    15241 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
    15301 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
    15361 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
    15421 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
    15481 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
    15541 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
    15601 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
    15661 cactataggg agacccaagc tggctagatt aagcgtctga tgagtccgtg aggacgaaac
    15721 ccggcgtacc gggtc
//
