LOCUS       sequence-139633-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BFPKRAB_PUFa in pmax-DEST expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1451_pmax-BFPKRAB_PUFa
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..57
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gactcgagcg gccgccactg tgctggatat caaccacttt gtacaagaaa gctgggtcga
       61 attcttaatt aatcacccta agtcaacacc gttcttcatg tagtacttct ccagcttggc
      121 cagaatgtgc ttgccatagg tgtacttacg aagagttgcg atgtggggcc ggatcttatg
      181 catgacgatc ttccgctggc ctggctccgc cacgtcaatc atcttctgga ccacgtagtt
      241 ggcatactgg tccttcatca tggtgtataa ggcactgtgg ggaccgtcgt tcatggtgca
      301 cacctcatcg atgagcacag cgcgctccgt acgtgaggcg tgagtaacac acttctccac
      361 aacattgctt gcaaatttgt gctgactcaa tacaagtaca ttgcctcgga tttctgctac
      421 aattttgctt ttatcctcag gacgaccgtg ctccagtaca tgttggatta cataatttcc
      481 atattgatcc tgtaccagct gctctgtgtg ctggtgaagc tcctctaaaa tagggagtgt
      541 ctggtcaggg agacagtgct ccaggattct ctgaatcact cggcagccat aaggatgtgt
      601 ggataaggca aatacctgtc ccttaaacgc atcgatgata aattgcaaag actggggctg
      661 tacacattca atgcatttct gaaccacgtg attgccattc tgatctttca cacacttcaa
      721 gacatggcca tctagttccc gaaccatctc attctgctgg tctgaaggaa taaactcaag
      781 agctttctcg ataacacggc tgccatacat ctgtagtgcc aatgacagga cgtggcctcg
      841 aatccgttct gccaaagcca gcttctgttc aagactgcca aattcaaaga acttctgaat
      901 gacgtaatta cc
//
