LOCUS       sequence-139634-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BFPKRAB_PUFa in pmax-DEST expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1451_pmax-BFPKRAB_PUFa
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             257..442
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             485..505
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc
       61 cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa
      121 ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga
      181 cctccctagc aaactggggc acaagcttaa tggcggtggc ggagggatgg atgctaagtc
      241 actaactgcc tggtcccgga cactggtgac cttcaaggat gtatttgtgg acttcaccag
      301 ggaggagtgg aagctgctgg acactgctca gcagatcgtg tacagaaatg tgatgctgga
      361 gaactataag aacctggttt ccttgggtta tcagcttact aagccagatg tgatcctccg
      421 gttggagaag ggagaagagc ccggcggttc cggcggaggg tcggggccgg ccgggatcct
      481 cccccccaag aaaaagagga aggtatctag aggccgcagc cgccttttgg aagattttcg
      541 aaacaaccgg taccccaatt tacaactgcg ggagattgct ggacatataa tggaattttc
      601 ccaagaccag catgggtcca gattcattca gctgaaactg gagcgtgcca caccagctga
      661 gcgccagctt gtcttcaatg aaatcctcca ggctgcctac caactcatgg tggatgtgtt
      721 tggtaattac gtcattcaga agttctttga atttggcagt cttgaacaga agctggcttt
      781 ggcagaacgg attcgaggcc acgtcctgtc attggcacta cagatgtatg gca
//
