LOCUS       sequence-13964-e        5364 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Fanning AS, Jameson BJ, Jesaitis LA, Anderson JM
  TITLE     The tight junction protein ZO-1 establishes a link between the 
            transmembrane protein occludin and the actin cytoskeleton.
  JOURNAL   J Biol Chem. 1998 Nov 6. 273(45):29745-53.
  PUBMED    9792688
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Nov 6. 273(45):29745-53."
COMMENT     Sequence Label: pCB6 ZO1myc
FEATURES             Location/Qualifiers
     source          1..5364
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1716..1724
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5326..5355
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ggtaccgaga gagacaagat gtccgccaga gctgcggccg ccaagagcac agcaatggag
       61 gaaacagcta tatgggaaca acatacagtg acgcttcaca gggctcctgg atttggattt
      121 ggaattgcaa tatctggtgg acgagataat cctcattttc agagtgggga aacgtcaata
      181 gtgatttcag atgtgctgaa aggaggacca gctgaaggac agctacagga aaatgaccga
      241 gttgcaatgg ttaacggagt ttcaatggat aatgttgaac atgcttttgc tgttcagcaa
      301 ctaaggaaaa gtgggaaaaa tgcaaaaatt acaattagaa ggaagaagaa agttcaaata
      361 ccagtaagtc gtcctgatcc tgaaccagta tctgataatg aagaagatag ttatgatgag
      421 gaaatacatg atccaagaag tggccggagt ggtgtggtta acagaaggag tgagaagatt
      481 tggccgaggg atagaagtgc aagtagagag aggagcttgt ccccgcggtc agacaggcgg
      541 tcagtggctt ccagccagcc tgctaaacct actaaagtca cactggtgaa atcccggaaa
      601 aatgaagaat atggtcttcg attggcaagc catatatttg ttaaggaaat ttcacaagat
      661 agtttggcag caagagatgg caatattcaa gaaggtgatg ttgtattgaa gataaatggt
      721 actgtgacag aaaatatgtc attgacagat gcaaagacat tgatagaaag gtctaaaggc
      781 aaattaaaaa tggtagttca aagagatgaa cgggctacgc tattgaatgt ccctgatctt
      841 tctgacagca tccactctgc taatgcctct gagagagacg acatttcaga aattcagtca
      901 ctggcatcag atcattctgg tcgatcacac gataggcctc cccgccgcag ccggtcacga
      961 tctcctgacc agcggtcaga gccttctgat cattccaggc actcgccgca gcagccaagc
     1021 aatggcagtc tccggagtag agatgaagag agaatttcta aacctggggc tgtctcaact
     1081 cctgtaaagc atgctgatga tcacacacct aaaacagtgg aagaagttac agttgaaaga
     1141 aatgagaaac aaacaccttc tcttccagaa ccaaagcctg tgtatgccca agttgggcaa
     1201 ccagatgtgg atttacctgt cagtccatct gatggtgtcc tacctaattc aactcatgaa
     1261 gatgggattc ttcggcccag catgaaattg gtaaaattca gaaaaggaga tagtgtgggt
     1321 ttgcggctgg ctggtggaaa tgatgttgga atatttgtag ctggcgttct agaagatagc
     1381 cctgcagcca aggaaggctt agaggaaggt gatcaaattc tcagggtaaa caacgtagat
     1441 tttacaaata tcataagaga agaagccgtc cttttcctgc ttgacctccc taaaggagaa
     1501 gaagtgacca tattggctca gaagaagaag gatgtttatc gtcgcattgt agaatcagat
     1561 gtaggagatt ctttctatat tagaacccat tttgaatatg aaaaggaatc tccctatgga
     1621 cttagtttta acaaaggaga ggtgttccgt gctgtggata ccttgtacaa tggaaaactg
     1681 ggctcttggc ttgctattcg aattggtaaa aatcataagg aggtagaacg aggcatcatc
     1741 cctaataaga acagagctga gcagctagcc agtgtacagt atacacttcc aaaaacagca
     1801 ggcggagacc gtgctgactt ctggagattc agaggtcttc gcagctccaa gagaaatctt
     1861 cgaaaaagca gagaggattt gtccgctcag cctgttcaaa caaagtttcc agcttatgaa
     1921 agagtggttc ttcgagaagc tggatttctg aggcctgtaa ccatttttgg accaatagct
     1981 gatgttgcca gagaaaagct ggcaagagaa gaaccagata tttatcaaat tgcaaagagt
     2041 gaaccacgag acgctggaac tgaccaacgt agctctggct atattcgcct gcatacaata
     2101 aagcaaatca tagatcaaga caaacatgct ttattagatg taacaccaaa tgcagttgat
     2161 cgtcttaact atgcccagtg gtatccaatt gttgtatttc ttaaccctga ttctaagcaa
     2221 ggagtaaaaa caatgagaat gaggttatgt ccagaatctc ggaaaagtgc caggaagtta
     2281 tacgagcgat ctcataaact tgctaaaaat aatcaccatc tttttacaac tacaattaac
     2341 ttaaattcaa tgaatgatgg ttggtatggt gcgctgaaag aagcagttca acaacagcaa
     2401 aaccagctgg tatgggtttc cgagggaaag gcggatggtg ctacaagtga tgaccttgat
     2461 ttgcatgatg atcgtctgtc ctacctgtca gctccaggta gtgaatactc aatgtatagc
     2521 acggacagta gacacacttc tgactatgaa gacacagaca cagaaggcgg ggcctacact
     2581 gatcaagaac tagatgaaac tcttaatgat gaggttggga ctccaccgga gtctgccatt
     2641 acacggtcct ctgagcctgt aagagaggac tcctctggaa tgcatcatga aaaccaaaca
     2701 tatcctcctt actcaccaca agcgcagcca caaccaattc atagaataga ctcccctgga
     2761 tttaagccag cctctcaaca gaaagcagaa gcttcatctc cagtccctta cctttcgcct
     2821 gaaacaaacc cagcatcatc aacctctgct gttaatcata atgtaaattt aactaatgtc
     2881 agactggagg agcccacccc agctccttcc acctcttact caccacaagc tgattcttta
     2941 agaacaccaa gtactgaggc agctcacata atgctaagag atcaagaacc atcattgtcg
     3001 tcgcatgtag atccaacaaa ggtgtataga aaggatccat atcccgagga aatgatgagg
     3061 cagaaccatg ttttgaaaca gccagccgtt agtcacccag ggcacaggcc agacaaagag
     3121 cctaatctga cctatgaacc ccaactccca tacgtagaga aacaagccag cagagacctc
     3181 gagcagccca catacagata cgagtcctca agctatacgg accagttttc tcgaaactat
     3241 gaacatcgtc tgcgatacga agatcgcgtc cccatgtatg aagaacagtg gtcatattat
     3301 gatgacaaac agccctaccc atctcggcca ccttttgata atcagcactc tcaagacctt
     3361 gactccagac agcatcccga agagtcctca gaacgagggt actttccacg ttttgaagag
     3421 ccagcccctc tgtcttacga cagcagacca cgttacgaac aggcacctag agcatccgcc
     3481 ctgcggcacg aagagcagcc agctcctggg tatgacacac atggtagact cagaccggaa
     3541 gcccagcccc acccttcagc agggcccaag cctgcagagt ccaagcagta ttttgagcaa
     3601 tattcacgca gttacgagca agtaccaccc caaggattta cctctagagc aggtcatttt
     3661 gagcctctcc atggtgctgc agctgtccct ccgctgatac cttcatctca gcataagcca
     3721 gaagctctgc cttcaaacac caaaccactg cctccacccc caactcaaac cgaagaagag
     3781 gaagatccag caatgaagcc acagtctgta ctcaccagag ttaagatgtt tgaaaacaaa
     3841 agatctgcat ccttagagac caagaaggat gtaaatgaca ctggcagttt taagcctcca
     3901 gaagtagcat ctaaaccttc aggtgctccc atcattggtc ccaaacccac ttctcagaat
     3961 caattcagtg aacatgacaa aactctgtac aggatcccag aacctcaaaa acctcaactg
     4021 aagccacctg aagatattgt tcggtccaat cattatgacc ctgaagaaga tgaagaatat
     4081 tatcgaaaac agctgtcata ctttgaccga agaagttttg agaataagcc tcctgcacac
     4141 attgctgcca gccatctctc cgagcctgca aagccagctc attctcagaa tcaatcaaat
     4201 ttttctagtt attcttcaaa gggaaagcct cctgaagctg atggtgtgga tagatcattt
     4261 ggcgagaaac gctatgaacc catccaggcc actccccctc ctcctccatt gccctcgcag
     4321 tatgcccagc catctcagcc tgtcaccagc gcgtctctcc acatacattc taagggagca
     4381 catggtgaag gtaattcagt gtcattggat tttcagaatt ccttagtgtc caaaccagac
     4441 ccacctccat ctcagaataa gccagcaact ttcagaccac caaaccgaga agatactgct
     4501 caggcagctt tctatcccca gaaaagtttt ccagataaag ccccagttaa tggaactgaa
     4561 cagactcaga aaacagtcac tccagcatac aatcgattca caccaaaacc atatacaagt
     4621 tctgcccgac catttgaacg caagtttgaa agtcctaaat tcaatcacaa tcttctgcca
     4681 agtgaaactg cacataaacc tgacttgtct tcaaaaactc ccacttctcc aaaaactctt
     4741 gtgaaatcgc acagtttggc acagcctcct gagtttgaca gtggagttga aactttctct
     4801 atccatgcag agaagcctaa atatcaaata aataatatca gcacagtgcc taaagctatt
     4861 cctgtgagtc cttcagctgt ggaagaggat gaagatgaag atggtcatac tgtggtggcc
     4921 acagcccgag gcatatttaa cagcaatggg ggcgtgctga gttccataga aactggtgtt
     4981 agtataatta tccctcaagg agccattccc gaaggagttg agcaggaaat ctatttcaag
     5041 gtctgccggg acaacagcat ccttccacct ttagataaag agaaaggtga aacactgctg
     5101 agtcctttgg tgatgtgtgg tccccatggc ctcaagttcc tgaagcctgt ggagctgcgc
     5161 ttaccacact gtgcgtccat gactcctgac ggttggtctt ttgctctaaa atcatccgac
     5221 tcctcgtcgg gtgatcctaa aacctggcaa aacaagtgtc ttcccggaga tccaaattat
     5281 ctcgttggag caaactgtgt ttctgtcctt attgaccact ttgccgagca aaagctgata
     5341 tctgaagagg acttgtaaac tagt
//
