LOCUS       sequence-139642-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PUFb_p65HSF1 in pmax-DEST expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1392_pmax-PUFb_p65HSF1
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..66
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcaggctccg aattcaccgg tgccgccacc atggggatcc tcccccccaa gaaaaagagg
       61 aaggtatcta gaggccgcag ccgccttttg gaagattttc gaaacaaccg gtaccccaat
      121 ttacaactgc gggagattgc tggacatata atggaatttt cccaagacca gcatgggtcc
      181 agattcattc agctgaaact ggagcgtgcc acaccagctg agcgccagct tgtcttcaat
      241 gaaatcctcc aggctgccta ccaactcatg gtggatgtgt ttggtaatta cgtcattcag
      301 aagttctttg aatttggcag tcttgaacag aagctggctt tggcagaacg gattcgaggc
      361 cacgtcctgt cattggcact acagatgtat ggctgccgtg ttatccagaa agctcttgag
      421 tttattcctt cagaccagca gaatgagatg gttcgggaac tagatggcca tgtcttgaag
      481 tgtgtgaaag atcagaatgg caatcacgtg gttcagaaat gcattgaatg tgtacagccc
      541 cagtctttgc aatttatcat cgatgcgttt aagggacagg tatttgcctt atccacacat
      601 ccttatggct gccgagtgat tcagagaatc ctggagcact gtctccctga ccagacactc
      661 cctattttag aggagcttca ccagcacaca gagcagctgg tacaggatca atatggaagt
      721 tatgtaatcg aacatgtact ggagcacggt cgtcctgagg ataaaagcaa aattgtagca
      781 gaaatccgag gcaatgtact tgtattgagt cagcacaaat ttgcaaacaa tgttgtgcag
      841 aagtgtgtta ctcacgcctc acgtacggag cgcgctgtgc tcatcgatg
//
