LOCUS       sequence-139643-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PUFb_p65HSF1 in pmax-DEST expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1392_pmax-PUFb_p65HSF1
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    30..54
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(79..450)
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
ORIGIN
        1 tcgagcggcc gccactgtgc tggatatcaa ccactttgta caagaaagct gggtcgaatt
       61 cttaattaat caatcgatgg agacagtggg gtccttggct ttgggaggct ccgagcctgt
      121 cagcagggag atggtggggt cctcggcgaa gccgtcccct tcggagaagt aggagccctc
      181 tcccagctca aacagcaccg gcaggtcgtt gctcccggtg tccacggagc cggggtccag
      241 caggaacagc ggctgcgctg tgtagtgcac cagctgcttc cctgaatccg ggctgctgtt
      301 ctctgcctcg ggaggcctgg ggggctcctg gggagacagg agctcttgga tactggccag
      361 gctgctgtca aggtcaggca ggctcatgtc gggcacggtc accgaggggc tgaacaggtc
      421 cagcagggca ctggtgtcca cgctgaagcc gcttccacct cctccctgcc cactagagga
      481 aatctgtgac agcagggcac taaagtccat atcagcgatg cttgagaagt cttcatctcc
      541 ggacagccca ttaggcaggc cgctggttcc caggggagtt ggagcggggt cggggggccg
      601 ctggctgccg gtcaccagcc gggtaatggc ttcggggtac tccatcagca ttggttcggc
      661 tgtactatga gacatggaca cgccctgatt cagcagctgc tgaaactcag agttgtccac
      721 ggaggccaga tctgtgaaca ctccgggatc ggtgctgttc cccagcagag ctcccaggtc
      781 ctcatcagcg tcgaactgca ggtgcagcag agcttcactc agagtcccct cgccggcctg
      841 tgtagacttg ggcactggag cgctcagtga ctggggtggt cctggggtca gcaca
//
