LOCUS       sequence-139645-        7329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified plasmid with a different backbone that yields higher of 
            AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Choudhury SR, Harris AF, Cabral DJ, Keeler AM, Sapp E, Ferreira JS, 
            Gray-Edwards HL, Johnson JA, Johnson AK, Su Q, Stoica L, DiFiglia M,
            Aronin N, Martin DR, Gao G, Sena-Esteves M
  TITLE     Widespread Central Nervous System Gene Transfer and Silencing After 
            Systemic Delivery of Novel AAV-AS Vector.
  JOURNAL   Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2015.231.
  PUBMED    26708003
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2015 Dec 28. doi: 10.1038/mt.2015.231."
COMMENT     Sequence Label: pGG-9.47_VP1,3 #2515
FEATURES             Location/Qualifiers
     source          1..7329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3935..3946
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4397..4417)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4397..4415)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4436..4452)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4436..4452)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4449..4471)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4460..4476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4484..4490,4491..4508,4509..4514))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4529..4550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4667..4684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4838..5426)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4918..4937)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5597..6388,6389..6457))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6220..6239
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6458..6562)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6588..7043)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6725..6746)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6937..6956
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7170..7192
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7184..7201
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7185..7201
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7211..7230
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7211..7229
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7258..7274
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7259..7275
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga gcatctgccc
       61 ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat
      121 tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga gaagctgcag
      181 cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggctct tttctttgtg
      241 caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac caccggggtg
      301 aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat tcagagaatt
      361 taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc
      421 gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt gctccccaaa
      481 acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag cgcctgtttg
      541 aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc gcagacgcag
      601 gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag atcaaaaact
      661 tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag
      721 cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc caactcgcgg
      781 tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac taaaaccgcc
      841 cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg gatttataaa
      901 attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct gggatgggcc
      961 acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac taccgggaag
     1021 accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt aaactggacc
     1081 aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg ggaggagggg
     1141 aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag caaggtgcgc
     1201 gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat cgtcacctcc
     1261 aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca ccagcagccg
     1321 ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga ctttgggaag
     1381 gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt ggttgaggtg
     1441 gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc cagtgacgca
     1501 gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac gtcagacgcg
     1561 gaagcttcga tcaactacgc ggacaggtac caaaacaaat gttctcgtca cgtgggcatg
     1621 aatctgatgc tgtttccctg cagacaatgc gagagactga atcagaattc aaatatctgc
     1681 ttcactcacg gtgtcaaaga ctgtttagag tgctttcccg tgtcagaatc tcaacccgtt
     1741 tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcacatcat gggaaaggtg
     1801 ccagacgctt gcactgcttg cgacctggtc aatgtggact tggatgactg tgtttctgaa
     1861 caataaatga cttaaaccag gtatggctgc cgatggttat cttccagatt ggctcgagga
     1921 caaccttagt gaaggaattc gcgagtggtg ggctttgaaa cctggagccc ctcaacccaa
     1981 ggcaaatcaa caacatcaag acaacgctcg aggtcttgtg cttccgggtt acaaatacct
     2041 tggacccggc aacggactcg acaaggggga gccggtcaac gcagcagacg cggcggccct
     2101 cgagcacgac aaggcctacg accagcagct caaggccgga gacaacccgt acctcaagta
     2161 caaccacgcc gacgccgagt tccaggagcg gctcaaagaa gatacgtctt ttgggggcaa
     2221 cctcgggcga gcagtcttcc aggccaaaaa gaggcttctt gaacctcttg gtctggttga
     2281 ggaagcggct aaggcggctc ctggaaagaa gaggcctgta gagcagtctc ctcaggaacc
     2341 ggactcctcc gcgggtattg gcaaatcggg tgcacagccc gctaaaaaga gactcaattt
     2401 cggtcagact ggcgacacag agtcagtccc agaccctcaa ccaatcggag aacctcccgc
     2461 agccccctca ggtgtgggat ctcttacaat ggcttcaggt ggtggcgcac cagtggcaga
     2521 caataacgaa ggtgccgatg gagtgggtag ttcctcggga aattggcatt gcgattccca
     2581 atggctgggg gacagagtca tcaccaccag cacccgaacc tgggccctgc ccacctacaa
     2641 caatcacctc tacaagcaaa tctccaacag cacatctgga ggatcttcaa atgacaacgc
     2701 ctacttcggc tacagcaccc cctgggggta ttttgacttc aacagattcc actgccactt
     2761 ctcaccacgt gactggcagc gactcatcaa caacaactgg ggattccggc ctaagcgact
     2821 caacttcaag ctcttcaaca ttcaggtcaa agaggttacg gacaacaatg gagtcaagac
     2881 catcgccaat aaccttacca gcacggtcca ggtcttcacg gactcagact atcagctccc
     2941 gtacgtgctc gggtcggctc acgagggctg cctcccgccg ttcccagcgg acgttttcat
     3001 gattcctcag tacgggtatc tgacgcttaa tgatggaagc caggccgtgg gtcgttcgtc
     3061 cttttactgc ctggaatatt tcccgtcgca aatgctaaga acgggtaaca acttccagtt
     3121 caactacgag tttgagaacg tacctttcca tagcagctac gctcacagcc aaagcctgga
     3181 ccgactaatg aatccactca tcgaccaata cttgtactat ctctcaaaga ctattaacga
     3241 ttctggacag aatcaacaaa cgctaaaatt cagtgtggcc ggacccagca acatggctgt
     3301 ccagggaaga aactacatac ctggacccag ctaccgacaa caacgtgtct caaccactgt
     3361 gactcaaaac aacaacagcg aatttgcttg gcctggagct tcttcttggg ctctcaatgg
     3421 acgtaatagc ttgatgaatc ctggacctgc tatggccagc cacaaagaag gagaggaccg
     3481 tttctttcct ttgtctggat ctttaatttt tggcaaacaa ggaactggaa gagacaacgt
     3541 ggatgcggac gaagtcatga taaccaacga agaagaaatt aaaactacta acccggtagc
     3601 aacggagtcc tatggacaag tggccataaa ccaccagagt gcccaagcac aggcgcagac
     3661 cggctgggtt caaaaccaag gaatacttcc gggtatggtt tggcaggaca gagatgtgta
     3721 cctgcaagga cccatttggg ccaaaattcc tcacacggac ggcaactttc acccttctcc
     3781 gctgatggga gggtttggaa tgaagcaccc gcctcctcag atcctcatca aaaacacacc
     3841 tgtacctgcg gatcctccaa cggccttcaa caaggacaag ctgaactctt tcatcaccca
     3901 gtattctact ggccaagtca gcgtggagat cgagtgggag ctgcagaagg aaaacagcaa
     3961 gcgctggaac ccggagatcc agtacacttc caactattac aagtctaata atgttgaatt
     4021 tgctgttaat actgaaggtg tatatagtga accccgcccc attggcacca gatacctgac
     4081 tcgtaatctg taattgcttg ttaatcaata aaccgtttaa ttcgtttcag ttgaactttg
     4141 gtctctgcga agggcgaatt cgtttaaacc tgcaggacta gagtcctgta ttagaggtca
     4201 cgtgagtgtt ttgcgacatt ttgcgacacc atgtggtcac gctgggtatt taagcccgag
     4261 tgagcacgca gggtctccat tttgaagcgg gaggtttgaa cgcgcagccg ccaagccgaa
     4321 ttctgcagat atccatcaca ctggcggccg ctcgactaga gcggccgcca ccgcggtgga
     4381 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     4441 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     4501 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     4561 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     4621 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     4681 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5521 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5581 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5641 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5701 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5761 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5821 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5881 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5941 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6001 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6061 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6121 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6181 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6241 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6301 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6361 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6421 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6481 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6541 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     6601 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     6661 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     6721 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     6781 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     6841 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     6901 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     6961 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7021 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
     7081 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     7141 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     7201 tgagcgcgcg taatacgact cactataggg cgaattgggt accgggcccc ccctcgatcg
     7261 aggtcgacgg tatcggggga gctcgcaggg tctccatttt gaagcgggag gtttgaacgc
     7321 gcagccgcc
//
