LOCUS       sequence-139646-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 expressed in pmax vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1445-pmax-dCas9
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          85..217
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     protein_bind    232..256
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             337..363
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             367..387
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgaaccgtca gatcactagt agctttattg cggtagttta tcacagttaa attgctaacg
       61 cagtcagtgc tcgactgatc acaggtaagt atcaaggtta caagacaggt ttaaggaggc
      121 caatagaaac tgggcttgtc gagacagaga agattcttgc gtttctgata ggcacctatt
      181 ggtcttactg acatccactt tgcctttctc tccacaggga aaaaacaatt gacaagtttg
      241 tacaaaaaag caggctccga attcaccggt gccgccacca tgatcgatgg tggcggtggt
      301 agcgggggag gcggctccgg gggcggaggc agtatgtacc catacgatgt tccagattac
      361 gcttcgccga agaaaaagcg caaggtcgaa gcgtccgaca agaagtacag catcggcctg
      421 gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
      481 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga
      541 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga
      601 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
      661 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
      721 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
      781 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
      841 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
      901 atcgagggcg acctgaaccc c
//
