LOCUS       sequence-139647-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  multiplex gRNA plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Port F, Bullock SL
  TITLE     Augmenting CRISPR applications in Drosophila with tRNA-flanked 
            sgRNAs.
  JOURNAL   Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 
            2016 Sep 5.
  PUBMED    27595403
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3972"; journalName: "Nat Methods"; date: "2016-10"; 
            volume: "13"; issue: "10"; pages: "852-4"
COMMENT     Sequence Label: pCFD5
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    288..673
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt ccatcatgat
       61 gaacgggtcg aggtggcggt agttgatccc ggcgaacgcg cggcgcaccg ggaagccctc
      121 gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg ggacgtgcga cggcgtcggc
      181 tggtgcggat acgcggggca gcgtcagcgg gttctcgacg gtcacggcgg gcatgtcgac
      241 aagccgaatt gatccactag aaggcctaat tcggtacact agttggccac gtaataagtg
      301 tgcgttgaat ttattcgcaa aaacattgca tattttcggc aaagtaaaat tttgttgcat
      361 accttatcaa aaaataagtg ctgcatactt tttagagaaa ccaaataatt ttttattgca
      421 tacccgtttt taataaaata cattgcatac cctcttttaa taaaaaatat tgcatacttt
      481 gacgaaacaa attttcgttg catacccaat aaaagattat tatattgcat acccgttttt
      541 aataaaatac attgcatacc ctcttttaat aaagaatatt gcatacgttg acgaaacaaa
      601 ttttcgttgc atacccaata aaagattatt atattgcata ccttttcttg ccataccatt
      661 tagccgatca attgagatct gaattctttt ttgctcacct gtgattgctc ctactcaaat
      721 acaaaaacat caaattttct gtcaataaag catatttatt tatatttatt ttacaggaaa
      781 gaattccttt taaagtgtat tttaacctat aatgaaaaac gattaaaaaa aatacataaa
      841 ataattcgaa aatttttgaa tagcccaggt tgataaaaat tcatttcata cgttttataa
      901 cttatgcccc taagtatttt ttgaccatag tgtttcaatt ctacattaat tttacagagt
      961 agaatgaaac gccacctact cagccaagag gcgaaaaggt tagctcgcca agcagagagg
     1021 gcgccagtgc tcactacttt ttataattct caacttcttt tt
//
