LOCUS       sequence-139649-        3323 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP-Ago2 lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Janas MM, Wang B, Harris AS, Aguiar M, Shaffer JM, Subrahmanyam YV, 
            Behlke MA, Wucherpfennig KW, Gygi SP, Gagnon E, Novina CD
  TITLE     Alternative RISC assembly: binding and repression of microRNA-mRNA 
            duplexes by human Ago proteins.
  JOURNAL   RNA. 2012 Nov;18(11):2041-55. doi: 10.1261/rna.035675.112. Epub 2012
            Sep 27.
  PUBMED    23019594
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.035675.112"; journalName: "RNA"; date: "2012-11"; 
            volume: "18"; issue: "11"; pages: "2041-55"
COMMENT     Sequence Label: pHAGE-eGFP-Ago2
FEATURES             Location/Qualifiers
     source          1..3323
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(15..17,18..20,21..731)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(60..81)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttttttgcgg ccgcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
       61 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg
      121 gcgatgccac caacggcaag ctgaccctga agttcatctg cacctccggc aagctgcccg
      181 tgccctggcc caccctcgtg accaccctgt cttacggcgt gcagtgcttc gcccgctacc
      241 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      301 agcgcaccat ctccttcaag gacgacggca gctacaggac ccgcgccgag gtgaagttcg
      361 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      421 acatcctggg gcacaagctg gagtacaaca tgaacgtgtg ggacgcgtat atcacggccg
      481 acaagcagaa gaacggcatc aaagcgaact tcaagatcga gcacaacgtc gaggacggcg
      541 gcgtgcagct cgccgacgcg taccagcaga acacccccat cggcgacggc tccgtgctgc
      601 tgcctgacaa ccactacctg agcttccaga gcaagctgtt caaagacccc aacgagcagc
      661 gcgatcacat ggtcctgctg gagttcgtta ccgccgccgg gatcactccc ggcatggacg
      721 agctgtacaa gggatccatg gaagcgggac cctcgggagc agctgcgggc gcttacctgc
      781 cccccctgca gcaggtgttc caggcacctc gccggcctgg cattggcact gtggggaaac
      841 caatcaagct cctggccaat tactttgagg tggacatccc taagatcgac gtgtaccact
      901 acgaggtgga catcaagccg gataagtgtc cccgtagagt caaccgggaa gtggtggaat
      961 acatggtcca gcatttcaag cctcagatct ttggtgatcg caagcctgtg tatgatggaa
     1021 agaagaacat ttacactgtc acagcactgc ccattggcaa cgaacgggtc gactttgagg
     1081 tgacaatccc tggggaaggg aaggatcgaa tctttaaggt ctccatcaag tggctagcca
     1141 ttgtgagctg gcgaatgctg catgaggccc tggtcagcgg ccagatccct gttcccttgg
     1201 agtctgtgca agccctggat gtggccatga ggcacctggc atccatgagg tacacccctg
     1261 tgggccgctc cttcttctca ccgcctgagg gctactacca cccgctgggg ggtgggcgcg
     1321 aggtctggtt cggctttcac cagtctgtgc gccctgccat gtggaagatg atgctcaaca
     1381 ttgatgtctc agccactgcc ttttataagg cacagccagt gattgagttc atgtgtgagg
     1441 tgctggacat caggaacata gatgagcagc ccaagcccct cacggactct cagcgcgttc
     1501 gcttcaccaa ggagatcaag ggcctgaagg tggaagtcac ccactgtgga cagatgaaga
     1561 ggaagtaccg cgtgtgtaat gttacccgtc gccctgctag ccatcagaca ttccccttac
     1621 agctggagag tggacagact gtggagtgca cagtggcaca gtatttcaag cagaaatata
     1681 accttcagct caagtatccc catctgccct gcctacaagt tggccaggaa caaaagcata
     1741 cctaccttcc cctagaggtc tgtaacattg tggctgggca gcgctgtatt aaaaagctga
     1801 ccgacaacca gacctcgacc atgataaagg ccacagctag atccgctcca gacagacagg
     1861 aggagatcag tcgcctgatg aagaatgcca gctacaactt agatccctac atccaggaat
     1921 ttgggatcaa agtgaaggat gacatgacgg aggtgacagg gcgagtgctg ccggcgccca
     1981 tcttgcagta cggcggccgg aaccgggcca ttgccacacc caatcagggt gtctgggaca
     2041 tgcgggggaa acagttctac aatgggattg agatcaaagt ctgggccatc gcctgcttcg
     2101 caccccaaaa acagtgtcga gaagaggtgc tcaagaactt cacagaccag ctgcggaaga
     2161 tttccaagga tgcggggatg cctatccagg gtcaaccttg tttctgcaaa tatgcacagg
     2221 gggcagacag cgtggagcct atgttccggc atctcaagaa cacctactca gggctgcagc
     2281 tcattattgt catcctgcca gggaagacgc cggtgtatgc tgaggtgaaa cgtgtcggag
     2341 atacactctt gggaatggct acgcagtgtg tgcaggtgaa gaacgtggtc aagacctcac
     2401 ctcagactct gtccaacctc tgcctcaaga tcaatgtcaa acttggtggc attaacaaca
     2461 tcctagtccc acaccagcgc tctgccgttt ttcaacagcc agtgatattc ctgggagcag
     2521 atgttacaca ccccccagca ggggatggga aaaaaccttc tatcacagca gtggtaggca
     2581 gtatggatgc ccaccccagc cgatactgtg ctactgtgcg ggtacagcga ccacggcaag
     2641 agatcattga agacttgtcc tacatggtgc gtgagctcct catccaattc tacaagtcca
     2701 cccgtttcaa gcctacccgc atcatcttct accgagatgg ggtgcctgaa ggccagctac
     2761 cccagatact ccactatgag ctactggcca ttcgtgatgc ctgcatcaaa ctggaaaagg
     2821 actaccagcc tgggatcact tatattgtgg tgcagaaacg ccatcacacc cgccttttct
     2881 gtgctgacaa gaatgagcga attgggaaga gtggtaacat cccagctggg accacagtgg
     2941 acaccaacat cacccaccca tttgagtttg acttctatct gtgcagccac gcaggcatcc
     3001 agggcaccag ccgaccatcc cattactatg ttctttggga tgacaaccgt ttcacagcag
     3061 atgagctcca gatcctgacg taccagctgt gccacactta cgtacgatgc acacgctctg
     3121 tctctatccc agcacctgcc tactatgccc gcctggtggc tttccgggca cgataccacc
     3181 tggtggacaa ggagcatgac agtggagagg ggagccacat atcggggcag agcaatgggc
     3241 gggaccccca ggccctggcc aaagccgtgc aggttcacca ggatactctg cgcaccatgt
     3301 acttcgctta aatcgataaa aaa
//
