LOCUS       sequence-13965-e        5376 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Fanning AS, Little BP, Rahner C, Utepbergenov D, Walther Z, Anderson
            JM
  TITLE     The unique-5 and -6 motifs of ZO-1 regulate tight junction strand 
            localization and scaffolding properties.
  JOURNAL   Mol Biol Cell. 2007 Mar . 18(3):721-31.
  PUBMED    17182847
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2007 Mar . 18(3):721-31."
COMMENT     Sequence Label: pTRE ZO1myc
FEATURES             Location/Qualifiers
     source          1..5376
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1722..1730
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5332..5361
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gaattcggta ccgagagaga caagatgtcc gccagagctg cggccgccaa gagcacagca
       61 atggaggaaa cagctatatg ggaacaacat acagtgacgc ttcacagggc tcctggattt
      121 ggatttggaa ttgcaatatc tggtggacga gataatcctc attttcagag tggggaaacg
      181 tcaatagtga tttcagatgt gctgaaagga ggaccagctg aaggacagct acaggaaaat
      241 gaccgagttg caatggttaa cggagtttca atggataatg ttgaacatgc ttttgctgtt
      301 cagcaactaa ggaaaagtgg gaaaaatgca aaaattacaa ttagaaggaa gaagaaagtt
      361 caaataccag taagtcgtcc tgatcctgaa ccagtatctg ataatgaaga agatagttat
      421 gatgaggaaa tacatgatcc aagaagtggc cggagtggtg tggttaacag aaggagtgag
      481 aagatttggc cgagggatag aagtgcaagt agagagagga gcttgtcccc gcggtcagac
      541 aggcggtcag tggcttccag ccagcctgct aaacctacta aagtcacact ggtgaaatcc
      601 cggaaaaatg aagaatatgg tcttcgattg gcaagccata tatttgttaa ggaaatttca
      661 caagatagtt tggcagcaag agatggcaat attcaagaag gtgatgttgt attgaagata
      721 aatggtactg tgacagaaaa tatgtcattg acagatgcaa agacattgat agaaaggtct
      781 aaaggcaaat taaaaatggt agttcaaaga gatgaacggg ctacgctatt gaatgtccct
      841 gatctttctg acagcatcca ctctgctaat gcctctgaga gagacgacat ttcagaaatt
      901 cagtcactgg catcagatca ttctggtcga tcacacgata ggcctccccg ccgcagccgg
      961 tcacgatctc ctgaccagcg gtcagagcct tctgatcatt ccaggcactc gccgcagcag
     1021 ccaagcaatg gcagtctccg gagtagagat gaagagagaa tttctaaacc tggggctgtc
     1081 tcaactcctg taaagcatgc tgatgatcac acacctaaaa cagtggaaga agttacagtt
     1141 gaaagaaatg agaaacaaac accttctctt ccagaaccaa agcctgtgta tgcccaagtt
     1201 gggcaaccag atgtggattt acctgtcagt ccatctgatg gtgtcctacc taattcaact
     1261 catgaagatg ggattcttcg gcccagcatg aaattggtaa aattcagaaa aggagatagt
     1321 gtgggtttgc ggctggctgg tggaaatgat gttggaatat ttgtagctgg cgttctagaa
     1381 gatagccctg cagccaagga aggcttagag gaaggtgatc aaattctcag ggtaaacaac
     1441 gtagatttta caaatatcat aagagaagaa gccgtccttt tcctgcttga cctccctaaa
     1501 ggagaagaag tgaccatatt ggctcagaag aagaaggatg tttatcgtcg cattgtagaa
     1561 tcagatgtag gagattcttt ctatattaga acccattttg aatatgaaaa ggaatctccc
     1621 tatggactta gttttaacaa aggagaggtg ttccgtgctg tggatacctt gtacaatgga
     1681 aaactgggct cttggcttgc tattcgaatt ggtaaaaatc ataaggaggt agaacgaggc
     1741 atcatcccta ataagaacag agctgagcag ctagccagtg tacagtatac acttccaaaa
     1801 acagcaggcg gagaccgtgc tgacttctgg agattcagag gtcttcgcag ctccaagaga
     1861 aatcttcgaa aaagcagaga ggatttgtcc gctcagcctg ttcaaacaaa gtttccagct
     1921 tatgaaagag tggttcttcg agaagctgga tttctgaggc ctgtaaccat ttttggacca
     1981 atagctgatg ttgccagaga aaagctggca agagaagaac cagatattta tcaaattgca
     2041 aagagtgaac cacgagacgc tggaactgac caacgtagct ctggctatat tcgcctgcat
     2101 acaataaagc aaatcataga tcaagacaaa catgctttat tagatgtaac accaaatgca
     2161 gttgatcgtc ttaactatgc ccagtggtat ccaattgttg tatttcttaa ccctgattct
     2221 aagcaaggag taaaaacaat gagaatgagg ttatgtccag aatctcggaa aagtgccagg
     2281 aagttatacg agcgatctca taaacttgct aaaaataatc accatctttt tacaactaca
     2341 attaacttaa attcaatgaa tgatggttgg tatggtgcgc tgaaagaagc agttcaacaa
     2401 cagcaaaacc agctggtatg ggtttccgag ggaaaggcgg atggtgctac aagtgatgac
     2461 cttgatttgc atgatgatcg tctgtcctac ctgtcagctc caggtagtga atactcaatg
     2521 tatagcacgg acagtagaca cacttctgac tatgaagaca cagacacaga aggcggggcc
     2581 tacactgatc aagaactaga tgaaactctt aatgatgagg ttgggactcc accggagtct
     2641 gccattacac ggtcctctga gcctgtaaga gaggactcct ctggaatgca tcatgaaaac
     2701 caaacatatc ctccttactc accacaagcg cagccacaac caattcatag aatagactcc
     2761 cctggattta agccagcctc tcaacagaaa gcagaagctt catctccagt cccttacctt
     2821 tcgcctgaaa caaacccagc atcatcaacc tctgctgtta atcataatgt aaatttaact
     2881 aatgtcagac tggaggagcc caccccagct ccttccacct cttactcacc acaagctgat
     2941 tctttaagaa caccaagtac tgaggcagct cacataatgc taagagatca agaaccatca
     3001 ttgtcgtcgc atgtagatcc aacaaaggtg tatagaaagg atccatatcc cgaggaaatg
     3061 atgaggcaga accatgtttt gaaacagcca gccgttagtc acccagggca caggccagac
     3121 aaagagccta atctgaccta tgaaccccaa ctcccatacg tagagaaaca agccagcaga
     3181 gacctcgagc agcccacata cagatacgag tcctcaagct atacggacca gttttctcga
     3241 aactatgaac atcgtctgcg atacgaagat cgcgtcccca tgtatgaaga acagtggtca
     3301 tattatgatg acaaacagcc ctacccatct cggccacctt ttgataatca gcactctcaa
     3361 gaccttgact ccagacagca tcccgaagag tcctcagaac gagggtactt tccacgtttt
     3421 gaagagccag cccctctgtc ttacgacagc agaccacgtt acgaacaggc acctagagca
     3481 tccgccctgc ggcacgaaga gcagccagct cctgggtatg acacacatgg tagactcaga
     3541 ccggaagccc agccccaccc ttcagcaggg cccaagcctg cagagtccaa gcagtatttt
     3601 gagcaatatt cacgcagtta cgagcaagta ccaccccaag gatttacctc tagagcaggt
     3661 cattttgagc ctctccatgg tgctgcagct gtccctccgc tgataccttc atctcagcat
     3721 aagccagaag ctctgccttc aaacaccaaa ccactgcctc cacccccaac tcaaaccgaa
     3781 gaagaggaag atccagcaat gaagccacag tctgtactca ccagagttaa gatgtttgaa
     3841 aacaaaagat ctgcatcctt agagaccaag aaggatgtaa atgacactgg cagttttaag
     3901 cctccagaag tagcatctaa accttcaggt gctcccatca ttggtcccaa acccacttct
     3961 cagaatcaat tcagtgaaca tgacaaaact ctgtacagga tcccagaacc tcaaaaacct
     4021 caactgaagc cacctgaaga tattgttcgg tccaatcatt atgaccctga agaagatgaa
     4081 gaatattatc gaaaacagct gtcatacttt gaccgaagaa gttttgagaa taagcctcct
     4141 gcacacattg ctgccagcca tctctccgag cctgcaaagc cagctcattc tcagaatcaa
     4201 tcaaattttt ctagttattc ttcaaaggga aagcctcctg aagctgatgg tgtggataga
     4261 tcatttggcg agaaacgcta tgaacccatc caggccactc cccctcctcc tccattgccc
     4321 tcgcagtatg cccagccatc tcagcctgtc accagcgcgt ctctccacat acattctaag
     4381 ggagcacatg gtgaaggtaa ttcagtgtca ttggattttc agaattcctt agtgtccaaa
     4441 ccagacccac ctccatctca gaataagcca gcaactttca gaccaccaaa ccgagaagat
     4501 actgctcagg cagctttcta tccccagaaa agttttccag ataaagcccc agttaatgga
     4561 actgaacaga ctcagaaaac agtcactcca gcatacaatc gattcacacc aaaaccatat
     4621 acaagttctg cccgaccatt tgaacgcaag tttgaaagtc ctaaattcaa tcacaatctt
     4681 ctgccaagtg aaactgcaca taaacctgac ttgtcttcaa aaactcccac ttctccaaaa
     4741 actcttgtga aatcgcacag tttggcacag cctcctgagt ttgacagtgg agttgaaact
     4801 ttctctatcc atgcagagaa gcctaaatat caaataaata atatcagcac agtgcctaaa
     4861 gctattcctg tgagtccttc agctgtggaa gaggatgaag atgaagatgg tcatactgtg
     4921 gtggccacag cccgaggcat atttaacagc aatgggggcg tgctgagttc catagaaact
     4981 ggtgttagta taattatccc tcaaggagcc attcccgaag gagttgagca ggaaatctat
     5041 ttcaaggtct gccgggacaa cagcatcctt ccacctttag ataaagagaa aggtgaaaca
     5101 ctgctgagtc ctttggtgat gtgtggtccc catggcctca agttcctgaa gcctgtggag
     5161 ctgcgcttac cacactgtgc gtccatgact cctgacggtt ggtcttttgc tctaaaatca
     5221 tccgactcct cgtcgggtga tcctaaaacc tggcaaaaca agtgtcttcc cggagatcca
     5281 aattatctcg ttggagcaaa ctgtgtttct gtccttattg accactttgc cgagcaaaag
     5341 ctgatatctg aagaggactt gtaaactagt tctaga
//
