LOCUS       sequence-139651-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA vector for AsCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Port F, Bullock SL
  TITLE     Augmenting CRISPR applications in Drosophila with tRNA-flanked 
            sgRNAs.
  JOURNAL   Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 
            2016 Sep 5.
  PUBMED    27595403
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3972"; journalName: "Nat Methods"; date: "2016-10"; 
            volume: "13"; issue: "10"; pages: "852-4"
COMMENT     Sequence Label: pCFD7
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        577..997
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
ORIGIN
        1 ggcgtgccct tgggctcccc gggcgcgtac tccacctcac ccatctggtc catcatgatg
       61 aacgggtcga ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg
      121 ccctcgaaac cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggct
      181 ggtgcggata cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca
      241 agccgaattg atccactaga aggcctaatt cggtacacta gttggccacg taataagtgt
      301 gcgttgaatt tattcgcaaa aacattgcat attttcggca aagtaaaatt ttgttgcata
      361 ccttatcaaa aaataagtgc tgcatacttt ttagagaaac caaataattt tttattgcat
      421 acccgttttt aataaaatac attgcatacc ctcttttaat aaagaatatt gcatacgttg
      481 acgaaacaaa ttttcgttgc atacccaata aaagattatt atattgcata ccttttcttg
      541 ccataccatt tagccgatca attgagatct gaattctttt ttgctcacct gtgattgctc
      601 ctactcaaat acaaaaacat caaattttct gtcaataaag catatttatt tatatttatt
      661 ttacaggaaa gaattccttt taaagtgtat tttaacctat aatgaaaaac gattaaaaaa
      721 aatacataaa ataattcgaa aatttttgaa tagcccaggt tgataaaaat tcatttcata
      781 cgttttataa cttatgcccc taagtatttt ttgaccatag tgtttcaatt ctacattaat
      841 tttacagagt agaatgaaac gccacctact cagccaagag gcgaaaaggt tagctcgcca
      901 agcagagagg gcgccagtgc tcactacttt ttataattct caacttcttt ttccagactc
      961 agttcgtata tatagaccta ttttcaattt aacgtcgggg ctttgagtgt gtgtagacat
     1021 caagcatcgg tggttcagtg gtagaatgct cgcctgccac gcgggcggcc cgggttcgat
     1081 tcccggccga
//
