LOCUS       sequence-139652-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression plasmid of AsCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Port F, Bullock SL
  TITLE     Augmenting CRISPR applications in Drosophila with tRNA-flanked 
            sgRNAs.
  JOURNAL   Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 
            2016 Sep 5.
  PUBMED    27595403
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3972"; journalName: "Nat Methods"; date: "2016-10"; 
            volume: "13"; issue: "10"; pages: "852-4"
COMMENT     Sequence Label: act-AsCpf1
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttttgccat ttctacatta ccaaataagg taaccaaaaa ctaatgggaa atccgcattc
       61 tttccattgc agcttacagg atcgatcccc gggaattcac catgacacag ttcgagggct
      121 ttaccaacct gtatcaggtg agcaagacac tgcggtttga gctgatccca cagggcaaga
      181 ccctgaagca catccaggag cagggcttca tcgaggagga caaggcccgc aatgatcact
      241 acaaggagct gaagcccatc atcgatcgga tctacaagac ctatgccgac cagtgcctgc
      301 agctggtgca gctggattgg gagaacctga gcgccgccat cgactcctat agaaaggaga
      361 aaaccgagga gacaaggaac gccctgatcg aggagcaggc cacatatcgc aatgccatcc
      421 acgactactt catcggccgg acagacaacc tgaccgatgc catcaataag agacacgccg
      481 agatctacaa gggcctgttc aaggccgagc tgtttaatgg caaggtgctg aagcagctgg
      541 gcaccgtgac cacaaccgag cacgagaacg ccctgctgcg gagcttcgac aagtttacaa
      601 cctacttctc cggcttttat gagaacagga agaacgtgtt cagcgccgag gatatcagca
      661 cagccatccc acaccgcatc gtgcaggaca acttccccaa gtttaaggag aattgtcaca
      721 tcttcacacg cctgatcacc gccgtgccca gcctgcggga gcactttgag aacgtgaaga
      781 aggccatcgg catcttcgtg agcacctcca tcgaggaggt gttttccttc cctttttata
      841 accagctgct gacacagacc cagatcgacc tgtataacca gctgctggga ggaatctctc
      901 gggaggcagg caccgagaag atcaagggcc tgaacgaggt gctgaatctg gccatccaga
      961 agaatgatga gacagcccac atcatcgcct ccctgccaca cagattcatc ccc
//
