LOCUS       sequence-139658-       15766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  unc-70 tension sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15766)
  AUTHORS   Krieg M, Dunn AR, Goodman MB
  TITLE     Mechanical control of the sense of touch by beta-spectrin.
  JOURNAL   Nat Cell Biol. 2014 Mar;16(3):224-33. doi: 10.1038/ncb2915. Epub 
            2014 Feb 23.
  PUBMED    24561618
REFERENCE   2  (bases 1 to 15766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2915"; 
            journalName: "Nat Cell Biol"; date: "2014-03"; volume: "16"; issue: 
            "3"; pages: "224-33"
COMMENT     Sequence Label: mg319
FEATURES             Location/Qualifiers
     source          1..15766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2093..2113
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(5592..5612)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5615..6322
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(6443..6445,6446..6448,6449..7159)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6488..6509)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6749..6768)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7096..7117
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    7161..7181
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(11232..11256)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11309..11327)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11323..11370
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(11736..11741,11742..11758,11759..11764))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(11791..11809)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11877..11981
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11982..12050,12051..12842)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12200..12219)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13013..13601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13502..13521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13755..13772
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13787..13929
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13855..13874)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14015..14037
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(14031..14222)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     15684..15703
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agatctcgat cccgcgaaat taatacgact cactataggg agaccacaac ggtttccctc
       61 tagatcacaa gtttgtacaa aaaagcaggc tatttttaat gctactcatt aagaacttac
      121 ttgacattag aactcaaaat ctaattgttg ttcggaatta atttcgaata catcaattta
      181 tcttattctc tcctcccgca atcataaacc accagaccag tagcatctgt aaagtgcgcg
      241 gccttcctcc acccatcact ctctcgtaac ctcaatgaaa tagtggcgcg ttcgatgagg
      301 gtatcctcct gcgtctgtgg tacacaaaac gctatttttt gcctgcaacc cgggggccct
      361 ctttctctct cggtaccctc cacccaatcg atcggatgtg tgccaaccct tcttgcctgc
      421 atcttttgcg cgcaccaacg gtcctctctg ccgctctctc tgactctccg acaatgctgc
      481 actctacgtt ctccacatca ttccgactcc gtctctccgt cgtcgcatcc gtccccgtcg
      541 tttagccaat cctccttttc ctcttcctcc tcctcgtttt cttctcgatt tcaattccag
      601 cagcagcagc actgtcgtcg cttttcgttc taggatctct acttctttat tgtgatattg
      661 ttttcttgtt gttcgcggtg agatagtaaa gaaaaagaga gaggcgccgc gcatcccgtt
      721 cgtcaaaacg atgttgccgt tacccaatat aacgattgtt gtcttttgcc attttgtagg
      781 aactgtcgag ttgtaaacaa catgcaaaat ttggaacatt ttttgaactg aggttcgcat
      841 ttcgttgttt atgaatcaaa atgttacttt ttttcggtga gaacggagat ttgcagttca
      901 cctggaacta ggttcttttt atttttattt tgtctacaaa ctgaatgttg tttagttttg
      961 ttatcgataa caatgcaatt ctcaaatttg aaattgtgga aacaactgca aggtgccaga
     1021 agttttgaat ctgaaaccac gacattatct ttttgaaact atttttttga atgaatattt
     1081 gcagttctaa aaagtaaatc gtcatttcaa aattgtttct catgcaaaaa taaaggcaag
     1141 atcaaatgtg tttttgaata gacctgcgtt tttcagatca gtagatcaat cagcgtccct
     1201 agaaatattc tcaaaatttt tctcgtgcca actcaaacat ttgctcccat attttcgtaa
     1261 tttcttatag tatgcttttt tcgtcgtctt ctcctgctac atgtattcag tgctaatcct
     1321 aatcccttct gctaatttta tccgctttcc catccacatc cacacacatt ctttttctct
     1381 ttttattggc tgctcccact cgacactcat ttgggccaca aggacttggg ggacccatgt
     1441 gtatgttcga gttgtggggc tctatgtgtg tgtgtgtgtg tgtgtgtttg agtgcgcctc
     1501 tacgcagttg tcgagggccc cagccggcgg tctcccccaa cggccactgc cctgcttcta
     1561 cgtctttttc ttcttgtcct ccacctcccc aaccactggg tgcccttgct tttgaaccaa
     1621 acgggcagtg ctcccttttc cttccactta tttgattcgt cccaatgttt gtgtctctca
     1681 ccctctcaat cttttcatta cttgaataca ttgctttcta aactttttct tccttctttt
     1741 ctgattcttc ttgttttcca cccttttcca attggcagag catgggagca aagggccctc
     1801 ctactaattg ccaatgctcc gttgttccac catttttttt gtgttgtttt ttgtcgtctc
     1861 ctctccatcc agccgtcgtg tatttttctc tacgcctcat gaactatttt catcaactcg
     1921 ccaattcata agcccccttg gcttaaatga atgcgagggt ccctttttct gtcaattttt
     1981 tttctcgcct ctgctcagca acactggaaa tcgagacagg tttttattaa tctatgattt
     2041 ttcagcaaaa tttcggcggg acttgcaacg gcgcgaaacg tcgtcggcaa tacaactttg
     2101 tatacaaaag ttgggtatgg ctacggtcga cggcaattca ggcgcctata cccaagatga
     2161 ggactatgat gacaactcgt ccgcccgcct cttcgaacgt tctcgtatca aggcactagc
     2221 agatgaacgt gaactcgtgc aaaagaaaac atttacaaaa tgggtcaact cacacttggt
     2281 tcgagtcagc tgcaaagtac aagacctcta catggatatg cgagacggaa aaatgctcct
     2341 ccgactcctc gcagttcttt ccggtgaacg tctgccgaaa ccgacgccgg gaaaaatgcg
     2401 tatccactgt cttgagaacg ttgagaaagg tttgcaattc ttgcgcaatc aacacgtgca
     2461 tctcgaaaac ttgggttcac acgatatcgt tgacggaaat tcacgcctca cgttgggtct
     2521 catctggacg atcatccttc gattccagat ccaagatatc acatttgaag acgctgacaa
     2581 tcacgagaca cgttcagcga aggaggcctt gttactgtgg tgccagatga aaactgctgg
     2641 atatccaaat gtcaacgtca agaacttctc aaccagttgg agagatggtc ttgcattcaa
     2701 tgcacttatt cataaacata gacctgatct cgtagactac gataatttac aaaaatcgaa
     2761 tgctctttac aatttgcaat ctgcttttga taccgcagaa aatcaattgg gattggccaa
     2821 gttcctcgat gccgaagatg tgaacgttga ccaacctgat gagaaatcca ttatcactta
     2881 tgtagttaca tattatcatt acttcaacaa actgaaacaa gataacatcc aaggaaaacg
     2941 tatcggaaaa gtgattaatg agttgatgga gaacgacaaa atgatcaatc gatacgaaac
     3001 tctttcatct gatttgctcg aatggatcaa tgccaagatc caacttctca acgaacgtca
     3061 ttttgagaat aatttggaag gtgttcaaag acagctcacc gagttcaacg actaccgtac
     3121 tcaggagaaa ccaccaaagt tcgatgaaaa gggagagctc gaagtacttc tgttcactct
     3181 tcaatcagct atgcgtgcca acaatcagag acctttcgtg ccgagagaag gaaaactgat
     3241 tgctgatatc aaccgagcat ggcaatccct tgagaaggca gaacacgaac gagaacttgt
     3301 cctgaaagag gaactcattc gtcaagagaa actcgaacaa ctcgcagccc gattcaacag
     3361 aaaggccgag atgcgagaga cttggctcac agaaaaccaa cggcttgtca gtcaggacaa
     3421 tttcggaaac gacttgtctt cggtggaagc tgccaccaaa aagcacgaag ccatcgagac
     3481 ggacatcttt gcttacgagg agcgtgttca agccgtcgtc gctgttgctg gagagcttga
     3541 agccgagaac taccatgatc aagccaaaat caatgagaga aaggagaatg ttcttcaact
     3601 ctggaactat cttttccaac ttcttttggc tcgcagagtt cgattggagt tgtcaatggc
     3661 cattcaaaag atcttccacg atatgcttct tactctcgat ctcatggatg atattaagag
     3721 cagactttta agcgaggatc ttggagccca tcttatggac gtagaagatt tgcttcaaaa
     3781 gcacgctctt cttgagtctg atattaatat tattggagaa cgagttaaca attctattgc
     3841 tcaagctcaa cgcttccgaa atccagatgg accagatgga agtggataca agccagttga
     3901 accaggaacc attgatgaga gaagtgatgt tctacaaaaa cgttacaagg agcttcttga
     3961 tttggctgct gaacgtaagc gtcgtctcga agacaacaag agactttgcc agttctggtg
     4021 ggatgttgct gagcttgaac atggtattaa ggaacaagag caagttcttt cgtccactga
     4081 tactggccgt gatattgtca ctgtttcgca tttgttggcc aaacataaga acgctgagaa
     4141 caaccttcgc gacttggaga agtacctcga tcgtcttgat gtttctggaa aagaattgca
     4201 agatgaaagc atcccaggaa gtgacaacat tcctccaagg cttgccgaga ttcgtgatta
     4261 catcaacaag ctcaaggaac tctctgcttc tcgcaaagaa agacttgccg gtggagtcga
     4321 atactaccaa ttcttcaccg acgccgacga tgtcgatcgc tacttgtatg atactctccg
     4381 cgtcatgtca tccgaagacg ttggaaaaga cgagggaact gttcaacttc tcctcaagaa
     4441 gcacgacgat gtccatgacg agctccagaa ctttgaccaa cacatcaagg ttcttcacgc
     4501 caaggccgag tctcttccac aggaagccag agaacatcca gatatcagac aacgattgga
     4561 cacaactctc aagcagaagg ccgaactcga aaacctttct caacttcgca agcagagact
     4621 tatcgatgct ctctccctgt acaagttgta ctcggatgcc gattcagtcg agtcttggat
     4681 cgatgagaag ggaaaacttt tggcaacatt ggtccctgga cgcgatatcg aagaggttga
     4741 gatcatgaag catcgtttcg atactcttga gcaagatatg aagaatcaag aagctaaggt
     4801 tactaacgtc aatgacttgg ctcgtcaatt gcttaatgtg gaacatccaa actccgacga
     4861 cattcttcac cgtcaaaaca agctcaacgc tcgctgggct caacttcgcg acatggtcga
     4921 tcaaaagaga aacgagctcg agagagctca ccgcctcgaa actttccgca tcgattgcca
     4981 agaaacagtt acttggatcg aggacaagac tcgtgtactc gaggattcgg acgcgttgac
     5041 caacgatctt tccggtgtca tgaaacttca aagaagattg tccatgatgg aacgcgattt
     5101 gggagcgatt caagccaagc tcgactcgct tcacaaagaa gccgatgaca tcgaaagaga
     5161 acgaccacaa gaagctcaag ccattcgtga agatatcaag agaatccacc aagtctggga
     5221 tatccttaac aagaaagttc gtgagcacga agctaaactc gatgaagctg gagatcttca
     5281 acgcttcctc cgtgatttgg atcacttcca agcctggctt actgccaccc agagacaagt
     5341 tgcctcagag gaagagccac aatccttggc tgaagctgag caacttctca atcaacacgc
     5401 tgccatccgt gaagagattg acggatacgc tgaggattac aagaagatgc gtgcaatggg
     5461 agatcgtgtc actcaagatc aaaccgatcc acaatacatg ttccttcgtc aacgtcttgc
     5521 tggacttcaa gaaggatggg aagagttgca gcgaatgtgg gacaacagac aacatttgct
     5581 ttctcaacac ccaacttttc tatacaaagt tgtaatggtg agcaagggcg aggagaccac
     5641 aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg
     5701 ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat
     5761 caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc
     5821 gttcgcctac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa
     5881 gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat
     5941 cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct
     6001 caagggcgag aacttccccc ccaacggccc cgtgatgcag aagaagacca ccggctggga
     6061 cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa
     6121 gctgctgctg gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa
     6181 gaaggcggtg aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca
     6241 cgacaaggac tacaacaagg tgaccgttta cgagagcgcc gtggcccgca actccaccga
     6301 cggcatggac gagctgtaca aggggccagg tggtgcaggg ccaggtggtg cagggccagg
     6361 tggtgcaggg ccaggtggtg cagggcccgg tggtgcaggt ccaggtggtg caggtccagg
     6421 tggtgcaggt ccaggtggtg ctatggtgag caagggcgag gagctgttca ccggggtggt
     6481 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     6541 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa
     6601 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc
     6661 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     6721 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     6781 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     6841 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     6901 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     6961 ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     7021 cgtgctgctg cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa
     7081 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     7141 catggacgag ctgtacaaga caactttgta taataaagtt gggtgcggac tcaaccttca
     7201 aatgttcctt cgcgatgcca aacaagcaga ggttatgctc tctcaacaag aaaactatct
     7261 cgccaaggac gatatcccac aatctttgga gcaagccgag aaccaactca agagacacca
     7321 agacttcatc accactatgg atgccaacga cgaaaagatt cgcgccgtcg gaatgttcgg
     7381 tgatcagctc tgccaagatg gtcactatgc cgctgacaag atccataaga aggctagaaa
     7441 cattgatgaa cgtcgtggag ccaaccgcga gaaggctcaa gaagtactca agaagctgaa
     7501 ggacgccttg tctctccaac aattcttgtc cgactgcgac gagctgagag aatggatcga
     7561 ggagaagatg atccgtgctc aagatgagac ttacagagac gccaagacta tcacctccaa
     7621 attcgtccga catcaagcat tccaatccga acttgctgct aacaaggaac gtcttgacca
     7681 acttaaacat gctgccatca accttggtga cgacaagcca gagtaccacg gaaccattga
     7741 tccacagatc gaggaactcg ctactcaatg ggatgagttg gaaaagacta cagaggagaa
     7801 gggacagaag ttgtttgatg ctaaccgcca acaactttac gtacaaagta ttgctgacat
     7861 gaaggaatgg gcaacccaac ttgagaacga gatgactcgt gaagaccaac caggagatct
     7921 taccactgtc aatgtggcca tgcaaaaaca acatttgatc gaaactgaaa tgatcaagaa
     7981 ggctcaacac attgatcaac tcatggagat ggagccacag ctcgaggaac ttcacccaga
     8041 cgagcttgaa aacattaagg ctcatcgttt ggcagttcaa gaacaacttc aaagactcca
     8101 ggctccattg gacgaccgac gtaaggcatt ggaacgcaag aaggccgcct tccaatttgg
     8161 tcgcgatgtt gatgacgaga aattgtggat ttctgagaga cttgttctcg ctaaagctca
     8221 aaatcttgga gaaagccttc cagattgtca tcgtctccag aagaacctac aacttctttc
     8281 caatgaaatc gacaaccacg aaccatggat caaccagatc tgtaataatg gtcaagagtt
     8341 gattgacgaa ggacatgcta acggaccagc attcgagaag aagattcaag aactgcgcag
     8401 tgcttggcaa gagttgaagg aagccgtcaa agacagaaag ggagatcttg gagaaagcga
     8461 gaaggctcat caattcttgt atgattgcgg agaggctgaa gcctggatga gcgaacaaga
     8521 attgtacatg atgcaagacg aaagaggaaa agacgagttc tcaaccaaga accaaatcaa
     8581 gaaacacgaa cgccttcaat ccgatattga caaatttgct gatacgatcc gtgctcttgc
     8641 caccaaggct cacaagttcg tcgaagagaa gtcaccactc accgaacaga tccaagttcg
     8701 tcaagctcaa atcgagaagc tctacgcagg tcttcaagat ttgtcgaagg agcgcagaaa
     8761 gagactcgaa gagaccttgg aactttacgc tcttcatcgt gaaatcgacg atcttcttca
     8821 atggatcgcc gacaaggaag ttgttgcagg aagtcaagag aacggacaag actacgaaca
     8881 cgtccaaatg cttcaagaga gattccaaca gtttgctcgt gacaccgaaa acatcggatc
     8941 cgaacgtgtt gccaatgcta atgatggatg tgatactctc attggtcacg gacacactga
     9001 cgctccgact atagcattgt ggaaggattc gttgaatgag gcctgggaga atcttctgga
     9061 gttgatggac actcgtgccc aaattctcga ggcttctaga cttcttcaca agttctacca
     9121 cgactgccgt gattgcttgt caagaatcat ggaaaagact catgctatgc ctgatgatct
     9181 tggacgtgac tcaagcagtg ttggtgcact ctccagaaaa catcagaact atctcaagga
     9241 tattgctgca attggagaac aagttgctca aatcgaaaga gatgccgcag agcttcgaga
     9301 tggttacgct ggagacaagg ctcttgatat cggatctcgc gaaagcgagg ttgtcaaggc
     9361 atggaggcat ctccgcggac tctgtgacgc ccgtacatct cgtctcatgg atacttctga
     9421 tcttttcaag ttcatgaata tggtccgtga tttgttgttg tggatggatg aggtgaagag
     9481 agaaatgaac tcacaagaac gtccgaagga tgtgagcgga gtcgagttgc tcatgaacaa
     9541 tcatcaatcg ctcaaggctg aaatcgatgc tcgcgaggag aacttcaacg cgtgcatctc
     9601 attgggacga gatctgctca acagaaaaca ctacgcatca agtgaaatcg agaagaagct
     9661 gatcaagttg acaaccgaaa gagccgaaat gatgcgcaga tgggaagatc gttgggaata
     9721 tcttcagctc attctggaag tgtaccaatt cgcccgcgac gctgctgtcg ccgaatcttg
     9781 gctcttcgcc caagaaccat atcttatttc gaaggaatac ggtagaaacc tcgaagaaac
     9841 catcaaactc atcaagaaac acgaggcgtt cgagaaatcg gcattcgcac aagaagaacg
     9901 tttcttggcg ctcgagaaat tgacaacgtt cgaattgaaa gaaactcaac accgcgagga
     9961 ggagaccgcc aagcgacgtg gtccagctca tattggaagt ccttctaggt caactccagc
    10021 cgcggaaaca tctttcggcg cccaggatga tggagccaaa caaggagaag cattcgaagg
    10081 aacgcttatc agaaagcaca cttacgagag tctcgaccgg aaagcggcta accgctcatg
    10141 ggagaaactt tacgcagtgt tgcggcagaa tgaactgtca ttctacaaag atccgaagca
    10201 tagagatgaa tctgtgcatg gagagccacc aatggcactg ccaggatgct ctgtcaacgt
    10261 ggcatcagac tatcaaaaga agaagaacgt actgtcgctg aggttaccca tcggcgccga
    10321 gtacctattc caatgcggta gcgaagaaga catgcagcga tggctcaccg agttgcaagt
    10381 cgccacgggg caagcccaac tggaagaggc tagtagatca cagacgctac cagcagaagg
    10441 cagtgccaca aagaagaagg gaggattttt ctctcgaggc aagaagtaac ctgaattctg
    10501 cagcatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat
    10561 gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat
    10621 ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa
    10681 atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat
    10741 aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga
    10801 taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg
    10861 tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa
    10921 tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt
    10981 tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt
    11041 cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat
    11101 cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag
    11161 tggagtagtg tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa
    11221 cataactgtt tacccagctt tcttgtacaa agtggtgatg atccggctgc taacaaagcc
    11281 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
    11341 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca
    11401 caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag
    11461 caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa
    11521 ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg
    11581 gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
    11641 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
    11701 actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg
    11761 tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
    11821 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
    11881 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    11941 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    12001 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    12061 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    12121 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    12181 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
    12241 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    12301 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    12361 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    12421 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    12481 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
    12541 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    12601 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    12661 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    12721 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    12781 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    12841 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    12901 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    12961 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    13021 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    13081 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    13141 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
    13201 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    13261 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    13321 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    13381 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    13441 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    13501 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    13561 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    13621 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    13681 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    13741 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
    13801 ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat
    13861 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
    13921 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
    13981 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
    14041 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
    14101 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
    14161 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
    14221 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
    14281 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
    14341 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
    14401 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
    14461 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
    14521 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
    14581 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
    14641 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca
    14701 acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc
    14761 tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt
    14821 ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc
    14881 tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc
    14941 atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc
    15001 cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt
    15061 cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag
    15121 cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt
    15181 agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg
    15241 tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg
    15301 acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg
    15361 ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga
    15421 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
    15481 gtcgatcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
    15541 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
    15601 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
    15661 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
    15721 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcg
//
