LOCUS       sequence-139660-        2942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  vector encoding a fusion protein between PML-IV and ER (estrogen 
            receptor).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Acevedo M, Vernier M, Mignacca L, Lessard F, Huot G, Moiseeva O, 
            Bourdeau V, Ferbeyre G
  TITLE     A CDK4/6-Dependent Epigenetic Mechanism Protects Cancer Cells from 
            PML-induced Senescence.
  JOURNAL   Cancer Res. 2016 Jun 1;76(11):3252-64. doi: 
            10.1158/0008-5472.CAN-15-2347. Epub 2016 Mar 29.
  PUBMED    27206849
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-15-2347"; journalName: "Cancer Res"; date: 
            "2016-06-1- 1"; volume: "76"; issue: "11"; pages: "3252-64"
COMMENT     Sequence Label: PML-ER-pBabe
FEATURES             Location/Qualifiers
     source          1..2942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2001..2936
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIXHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2049..2942
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIXHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
ORIGIN
        1 ggatccagat ctactagtaa cggccgccag tgtgctggaa ttgatctaaa ccgagaatcg
       61 aaactaagct ggggtccatg gagcctgcac ccgcccgatc tccgaggccc cagcaggacc
      121 ccgcccggcc ccaggagccc accatgcctc cccccgagac cccctctgaa ggccgccagc
      181 ccagccccag ccccagccct acagagcgag cccccgcttc ggaggaggag ttccagtttc
      241 tgcgctgcca gcaatgccag gcggaagcca agtgcccgaa gctgctgcct tgtctgcaca
      301 cgctgtgctc aggatgcctg gaggcgtcgg gcatgcagtg ccccatctgc caggcgccct
      361 ggcccctagg tgcagacaca cccgccctgg ataacgtctt tttcgagagt ctgcagcggc
      421 gcctgtcggt gtaccggcag attgtggatg cgcaggctgt gtgcacccgc tgcaaagagt
      481 cggccgactt ctggtgcttt gagtgcgagc agctcctctg cgccaagtgc ttcgaggcac
      541 accagtggtt cctcaagcac gaggcccggc ccctagcaga gctgcgcaac cagtcggtgc
      601 gtgagttcct ggacggcacc cgcaagacca acaacatctt ctgctccaac cccaaccacc
      661 gcacccctac gctgaccagc atctactgcc gaggatgttc caagccgctg tgctgctcgt
      721 gcgcgctcct tgacagcagc cacagtgagc tcaagtgcga catcagcgca gagatccagc
      781 agcgacagga ggagctggac gccatgacgc aggcgctgca ggagcaggat agtgcctttg
      841 gcgcggttca cgcgcagatg cacgcggccg tcggccagct gggccgcgcg cgtgccgaga
      901 ccgaggagct gatccgcgag cgcgtgcgcc aggtggtagc tcacgtgcgg gctcaggagc
      961 gcgagctgct ggaggctgtg gacgcgcggt accagcgcga ctacgaggag atggccagtc
     1021 ggctgggccg cctggatgct gtgctgcagc gcatccgcac gggcagcgcg ctggtgcaga
     1081 ggatgaagtg ctacgcctcg gaccaggagg tgctggacat gcacggtttc ctgcgccagg
     1141 cgctctgccg cctgcgccag gaggagcccc agagcctgca agctgccgtg cgcaccgatg
     1201 gcttcgacga gttcaaggtg cgcctgcagg acctcagctc ttgcatcacc caggggaaag
     1261 atgcagctgt atccaagaaa gccagcccag aggctgccag cactcccagg gaccctattg
     1321 acgttgacct gcccgaggag gcagagagag tgaaggccca ggttcaggcc ctggggctgg
     1381 ctgaagccca gcctatggct gtggtacagt cagtgcccgg ggcacacccc gtgccagtgt
     1441 acgccttctc catcaaaggc ccttcctatg gagaggatgt ctccaataca acgacagccc
     1501 agaagaggaa gtgcagccag acccagtgcc ccaggaaggt catcaagatg gagtctgagg
     1561 aggggaagga ggcaaggttg gctcggagct ccccggagca gcccaggccc agcacctcca
     1621 aggcagtctc accaccccac ctggatggac cgcctagccc caggagcccc gtcataggaa
     1681 gtgaggtctt cctgcccaac agcaaccacg tggccagtgg cgccggggag gcagaggaac
     1741 gcgttgtggt gatcagcagc tcggaagact cagatgccga aaactcgtcc tcccgagagc
     1801 tggatgacag cagcagtgag tccagtgacc tccagctgga aggccccagc accctcaggg
     1861 tcctggacga gaaccttgct gacccccaag cagaagacag acctctggtt ttctttgacc
     1921 tcaagattga caatgaaagt gggttctcct ggggctaccc ccaccccttt ctaatctcga
     1981 gcccgaattc cgatccatct gctggagaca tgagagctgc caacctttgg ccaagcccgc
     2041 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
     2101 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
     2161 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
     2221 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
     2281 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
     2341 tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
     2401 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
     2461 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
     2521 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
     2581 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
     2641 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcangc
     2701 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
     2761 tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgccc actagccgtg
     2821 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
     2881 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagtct
     2941 ga
//
