LOCUS       sequence-139663-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for C-terminal eGFP tagging, Puromycin-resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pSK23
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(2..4,5..7,8..721)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2..4,5..7,8..718)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(47..68)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(308..327)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     655..676
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(728..761)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
       61 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      121 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      181 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      241 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      301 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      361 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      421 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
      481 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      541 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      601 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      661 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
      721 aactagtgaa gttcctatac tttctagaga ataggaactt caaataaaca tatgggcgcg
      781 ccctgttgga atatactatt caacctacaa aaataacgtt aaacaacact actttatatt
      841 tgatatgaat gg
//
