LOCUS       sequence-139664-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for C-terminal eGFP tagging, Puromycin-resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pSK23
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(12..45)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 agtaaactag tgaagttcct atactttcta gagaatagga acttcaaata aacatatggg
       61 cgcgccctgt tggaatatac tattcaacct acaaaaataa cgttaaacaa cactacttta
      121 tatttgatat gaatggccac accttttatg ccataaaaca tattgtaaga gaataccact
      181 ctttttattc cttctttcct tcttgtacgt tttttgctgt gagtaggtcg tggtgctggt
      241 gttgcagttg aaataactta aaatataaat cataaaactc aaacataaac ttgactattt
      301 atttatttat taagaaagga ggtaccggtg gcgcgccatg accgagtaca agcctacagt
      361 gcgactggcc acacgcgatg acgttcccag agccgttcgg acgttggctg ccgcgtttgc
      421 ggactatccc gcaactcgcc atactgtgga ccctgatcgc cacatcgaga gggtgactga
      481 gcttcaggag ctgttcctga ccagggtagg gctggacata ggcaaggtct gggtggccga
      541 cgatggagcc gctgttgcgg tgtggactac cccagagagc gtggaagcag gggctgtctt
      601 tgccgaaatt gggccaagga tggccgaact gtctgggtcc cgtttggctg cccaacagca
      661 gatggaagga ctgcttgccc cacatcgacc caaggagcct gcatggttcc tcgctaccgt
      721 gggtgtgagc ccagatcacc agggcaaagg tctgggatca gccgtcgttc tgcctggcgt
      781 cgaggctgct gagagagcag gagtaccggc attcctcgaa accagtgctc cacggaacct
      841 cccgttctac gagagactgg g
//
