LOCUS       sequence-139666-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for C-terminal TEV-site + V5-tag, Puromycin-Resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pSK32
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..72
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(82..115)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 aacctgtatt ttcagggcgg ttccggatct ggcaagccga tcccgaatcc gctgttaggc
       61 ctggatagca cctgaactag tgaagttcct atactttcta gagaatagga acttcaaata
      121 aacatatggg cgcgccctgt tggaatatac tattcaacct acaaaaataa cgttaaacaa
      181 cactacttta tatttgatat gaatggccac accttttatg ccataaaaca tattgtaaga
      241 gaataccact ctttttattc cttctttcct tcttgtacgt tttttgctgt gagtaggtcg
      301 tggtgctggt gttgcagttg aaataactta aaatataaat cataaaactc aaacataaac
      361 ttgactattt atttatttat taagaaagga ggtaccggtg gcgcgccatg accgagtaca
      421 agcctacagt gcgactggcc acacgcgatg acgttcccag agccgttcgg acgttggctg
      481 ccgcgtttgc ggactatccc gcaactcgcc atactgtgga ccctgatcgc cacatcgaga
      541 gggtgactga gcttcaggag ctgttcctga ccagggtagg gctggacata ggcaaggtct
      601 gggtggccga cgatggagcc gctgttgcgg tgtggactac cccagagagc gtggaagcag
      661 gggctgtctt tgccgaaatt gggccaagga tggccgaact gtctgggtcc cgtttggctg
      721 cccaacagca gatggaagga ctgcttgccc cacatcgacc caaggagcct gcatggttcc
      781 tcgctaccgt gggtgtgagc ccagatcacc agggcaaagg tctgggatca gccgtcgttc
      841 tgcctggcgt cgaggctgct gagagagcag gagtaccgg
//
