LOCUS       sequence-139667-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for insertion of attP site (C-terminal tag plasmid design),
            Blasticidin-Resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pSK15
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(40..73)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             350..748
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(797..830)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ctggggtaac ctttgagttc tctcagttgg gggactagtg aagttcctat actttctaga
       61 gaataggaac ttcaaataaa catatgctgt tggaatatac tattcaacct acaaaaataa
      121 cgttaaacaa cactacttta tatttgatat gaatggccac accttttatg ccataaaaca
      181 tattgtaaga gaataccact ctttttattc cttctttcct tcttgtacgt tttttgctgt
      241 gagtaggtcg tggtgctggt gttgcagttg aaataactta aaatataaat cataaaactc
      301 aaacataaac ttgactattt atttatttat taagaaagga ggtaccggta tggccaagcc
      361 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
      421 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
      481 tggtgtcaat gtatatcatt ttactggggg accttgcgca gaactcgtgg tgctgggcac
      541 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
      601 gggcatcttg agcccctgcg gacggtgccg acaggttctt ctcgatctgc atcctgggat
      661 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
      721 gccctctggt tatgtgtggg agggctagaa attattaaaa ataaaacttt aaaaataaat
      781 ttgtttctaa catatggaag ttcctatact ttctagagaa taggaacttc atctttctag
      841 aagatctcct acaatattct cagctgccat gggaa
//
