LOCUS       sequence-139669-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template base vector for N-terminal tagging, inducible Blasticidin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pKF292
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    450..483
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 catgtcaaaa gagacgtctt ttgttaagaa tgctgaggaa cttgcaaagc aaaaaatgga
       61 tgctattaac cctgaacttt cttcaaaatt taaattttta ataaaattcc tgtctcagtt
      121 tcctgaagct tgctctaaac ctcgttcaaa aaaaatgcag aataaagttg gtcaagagga
      181 acatattgaa tatttagctc gtagttttca tgagagtcga ttgccaagaa aacccacgcc
      241 acctacaacg gttcctgatg aggtggttag catagttctt aatataagtt ttaatataca
      301 gcctgaaaat cttgagagaa taaaagaaga acatcgattt tccatggcag ctgagaatat
      361 tgtaggagat cttctagaaa gatactagta ccgccgcttg gagcagctgg agaggtaccg
      421 aattcaccgg tggatcccat tttggattag aagttcctat tctctagaaa gtataggaac
      481 ttccctaggt ttgtgttaaa atattttgtt gttgattata ttaaaataaa cttgattcac
      541 ttagccgtta gctagcactg tttgatgatt gtcgactggc ggtccagcat tcttatagag
      601 ctttcactgc tttgcgcacc aaaaaaatca attcctgtta tcagggcgtg tgcaaatcgc
      661 ttatcatcgc cgggcagaaa aagcattatg cttttgcgca cgccgatcga cttgcacacg
      721 gctttcttat cttatcgggc accacggccc acagtttcga ttggactgga tcgggtataa
      781 agaggaggat ttcccaacca gaaggcatca acaagttcaa taagctt
//
