LOCUS       sequence-139670-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template base vector for N-terminal tagging, inducible Blasticidin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pKF292
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(400..433)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 cctaggcttg ttgatttgtt ttttgtttat ttactgcggt aattcctagt cttagaagct
       61 tattgaactt gttgatgcct tctggttggg aaatcctcct ctttataccc gatccagtcc
      121 aatcgaaact gtgggccgtg gtgcccgata agataagaaa gccgtgtgca agtcgatcgg
      181 cgtgcgcaaa agcataatgc tttttctgcc cggcgatgat aagcgatttg cacacgccct
      241 gataacagga attgattttt ttggtgcgca aagcagtgaa agctctataa gaatgctgga
      301 ccgccagtcg acaatcatca aacagtgcta gctaacggct aagtgaatca agtttatttt
      361 aatataatca acaacaaaat attttaacac aaacctaggg aagttcctat actttctaga
      421 gaataggaac ttctaatcca aaatgggatc caccggtgaa ttcggtacct ctccagctgc
      481 tccaagcggc ggtactagta tctttctaga agatctccta caatattctc agctgccatg
      541 gaaaatcgat gttcttcttt tattctctca agattttcag gctgtatatt aaaacttata
      601 ttaagaacta tgctaaccac ctcatcagga accgttgtag gtggcgtggg ttttcttggc
      661 aatcgactct catgaaaact acgagctaaa tattcaatat gttcctcttg accaacttta
      721 ttctgcattt tttttgaacg aggtttagag caagcttcag gaaactgaga caggaatttt
      781 attaaaaatt taaattttga agaaagttca gggttaatag catccatttt
//
