LOCUS       sequence-139671-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal 3x FLAG tag, inducible Blasticidin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pKF293
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(406..439)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 catatgccta ggcttgttga tttgtttttt gtttatttac tgcggtaatt cctagtctta
       61 gaagcttatt gaacttgttg atgccttctg gttgggaaat cctcctcttt atacccgatc
      121 cagtccaatc gaaactgtgg gccgtggtgc ccgataagat aagaaagccg tgtgcaagtc
      181 gatcggcgtg cgcaaaagca taatgctttt tctgcccggc gatgataagc gatttgcaca
      241 cgccctgata acaggaattg atttttttgg tgcgcaaagc agtgaaagct ctataagaat
      301 gctggaccgc cagtcgacaa tcatcaaaca gtgctagcta acggctaagt gaatcaagtt
      361 tattttaata taatcaacaa caaaatattt taacacaaac ctagggaagt tcctatactt
      421 tctagagaat aggaacttct aatccaaaat gggatccacc ggtgaattcg actacaagga
      481 ccacgacggc gactacaagg accacgacat cgactacaag gacgacgaca agggtacctc
      541 tccagctgct ccaagcggcg gtactagtat ctttctagaa gatctcctac aatattctca
      601 gctgccatgg aaaatcgatg ttcttctttt attctctcaa gattttcagg ctgtatatta
      661 aaacttatat taagaactat gctaaccacc tcatcaggaa ccgttgtagg tggcgtgggt
      721 tttcttggca atcgactctc atgaaaacta cgagctaaat attcaatatg ttcctcttga
      781 ccaactttat tctgcatttt ttttgaacga ggtttagagc aagcttcagg aaactgagac
      841 aggaattt
//
