LOCUS       sequence-139675-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal 1x Strep tag, inducible Blasticidin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pKF294
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(432..455)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    480..513
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 catgtcaaaa gagacgtctt ttgttaagaa tgctgaggaa cttgcaaagc aaaaaatgga
       61 tgctattaac cctgaacttt cttcaaaatt taaattttta ataaaattcc tgtctcagtt
      121 tcctgaagct tgctctaaac ctcgttcaaa aaaaatgcag aataaagttg gtcaagagga
      181 acatattgaa tatttagctc gtagttttca tgagagtcga ttgccaagaa aacccacgcc
      241 acctacaacg gttcctgatg aggtggttag catagttctt aatataagtt ttaatataca
      301 gcctgaaaat cttgagagaa taaaagaaga acatcgattt tccatggcag ctgagaatat
      361 tgtaggagat cttctagaaa gatactagta ccgccgcttg gagcagctgg agaggtaccg
      421 aattcaccgg ttttttcgaa ctgcgggtgg ctccaagcgc tggatcccat tttggattag
      481 aagttcctat tctctagaaa gtataggaac ttccctaggt ttgtgttaaa atattttgtt
      541 gttgattata ttaaaataaa cttgattcac ttagccgtta gctagcactg tttgatgatt
      601 gtcgactggc ggtccagcat tcttatagag ctttcactgc tttgcgcacc aaaaaaatca
      661 attcctgtta tcagggcgtg tgcaaatcgc ttatcatcgc cgggcagaaa aagcattatg
      721 cttttgcgca cgccgatcga cttgcacacg gctttcttat cttatcgggc accacggccc
      781 acagtttcga ttggactgga tcgggtataa agaggaggat ttcccaacca gaagg
//
