LOCUS       sequence-139676-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal 1x Strep tag, inducible Blasticidin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pKF294
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(402..435)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             460..483
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 tgcctaggct tgttgatttg ttttttgttt atttactgcg gtaattccta gtcttagaag
       61 cttattgaac ttgttgatgc cttctggttg ggaaatcctc ctctttatac ccgatccagt
      121 ccaatcgaaa ctgtgggccg tggtgcccga taagataaga aagccgtgtg caagtcgatc
      181 ggcgtgcgca aaagcataat gctttttctg cccggcgatg ataagcgatt tgcacacgcc
      241 ctgataacag gaattgattt ttttggtgcg caaagcagtg aaagctctat aagaatgctg
      301 gaccgccagt cgacaatcat caaacagtgc tagctaacgg ctaagtgaat caagtttatt
      361 ttaatataat caacaacaaa atattttaac acaaacctag ggaagttcct atactttcta
      421 gagaatagga acttctaatc caaaatggga tccagcgctt ggagccaccc gcagttcgaa
      481 aaaaccggtg aattcggtac ctctccagct gctccaagcg gcggtactag tatctttcta
      541 gaagatctcc tacaatattc tcagctgcca tggaaaatcg atgttcttct tttattctct
      601 caagattttc aggctgtata ttaaaactta tattaagaac tatgctaacc acctcatcag
      661 gaaccgttgt aggtggcgtg ggttttcttg gcaatcgact ctcatgaaaa ctacgagcta
      721 aatattcaat atgttcctct tgaccaactt tattctgcat tttttttgaa cgaggtttag
      781 agcaagcttc aggaaactga gacaggaatt ttattaa
//
