LOCUS       sequence-139677-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of sgRNA targeting LINE-1 and TagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Arora R, Bodak M, Penouty L, Hackman C, Ciaudo C
  TITLE     Sequestration of LINE-1 in cytosolic aggregates by MOV10 restricts 
            retrotransposition.
  JOURNAL   EMBO Rep. 2022 Jul 20:e54458. doi: 10.15252/embr.202154458.
  PUBMED    35856394
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Jul 20:e54458. doi: 10.15252/embr.202154458."
COMMENT     Sequence Label: pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     109..128
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        206..281
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(339..362)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
       61 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      121 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      181 aacacccaga gaacctgaca gcttcgtttt agagctagaa atagcaagtt aaaataaggc
      241 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttgg atccaattct
      301 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
      361 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
      421 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
      481 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
      541 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
      601 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
      661 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
      721 cctccggagg cccggcattc tgcacgcttc aaa
//
