LOCUS       sequence-139680-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal Strep-8xHis-Strep-TEV site tag, inducible 
            Blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pKF290
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(402..435)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             460..483
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             490..513
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             520..543
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             556..576
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tgcctaggct tgttgatttg ttttttgttt atttactgcg gtaattccta gtcttagaag
       61 cttattgaac ttgttgatgc cttctggttg ggaaatcctc ctctttatac ccgatccagt
      121 ccaatcgaaa ctgtgggccg tggtgcccga taagataaga aagccgtgtg caagtcgatc
      181 ggcgtgcgca aaagcataat gctttttctg cccggcgatg ataagcgatt tgcacacgcc
      241 ctgataacag gaattgattt ttttggtgcg caaagcagtg aaagctctat aagaatgctg
      301 gaccgccagt cgacaatcat caaacagtgc tagctaacgg ctaagtgaat caagtttatt
      361 ttaatataat caacaacaaa atattttaac acaaacctag ggaagttcct atactttcta
      421 gagaatagga acttctaatc caaaatggga tccagcgctt ggagccaccc gcagttcgag
      481 aaaggtggac atcaccacca tcaccatcat cacggatcgt ggagccaccc gcagttcgaa
      541 aaaaccggtg aattcgaaaa cctgtatttt cagggcgaat tcggtacctc tccagctgct
      601 ccaagcggcg gtactagtat ctttctagaa gatctcctac aatattctca gctgccatgg
      661 aaaatcgatg ttcttctttt attctctcaa gattttcagg ctgtatatta aaacttatat
      721 taagaactat gctaaccacc tcatcaggaa ccgttgtagg tggcgtgggt tttcttggca
      781 atcgactctc atgaaaacta cgagctaaat attcaatatg ttcctcttga ccaactttat
      841 tctgcatttt ttttgaacga ggtttagagc aagcttcagg aaa
//
