LOCUS       sequence-139681-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal Strep-8xHis-Strep-TEV site tag, inducible 
            Blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pKF290
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(427..447)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(460..483)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(490..513)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(520..543)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    568..601
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 acatgtcaaa agagacgtct tttgttaaga atgctgagga acttgcaaag caaaaaatgg
       61 atgctattaa ccctgaactt tcttcaaaat ttaaattttt aataaaattc ctgtctcagt
      121 ttcctgaagc ttgctctaaa cctcgttcaa aaaaaatgca gaataaagtt ggtcaagagg
      181 aacatattga atatttagct cgtagttttc atgagagtcg attgccaaga aaacccacgc
      241 cacctacaac ggttcctgat gaggtggtta gcatagttct taatataagt tttaatatac
      301 agcctgaaaa tcttgagaga ataaaagaag aacatcgatt ttccatggca gctgagaata
      361 ttgtaggaga tcttctagaa agatactagt accgccgctt ggagcagctg gagaggtacc
      421 gaattcgccc tgaaaataca ggttttcgaa ttcaccggtt ttttcgaact gcgggtggct
      481 ccacgatccg tgatgatggt gatggtggtg atgtccacct ttctcgaact gcgggtggct
      541 ccaagcgctg gatcccattt tggattagaa gttcctattc tctagaaagt ataggaactt
      601 ccctaggttt gtgttaaaat attttgttgt tgattatatt aaaataaact tgattcactt
      661 agccgttagc tagcactgtt tgatgattgt cgactggcgg tccagcattc ttatagagct
      721 ttcactgctt tgcgcaccaa aaaaatcaat tcctgttatc agggcgtgtg caaatcgctt
      781 atcatcgccg ggcagaa
//
