LOCUS       sequence-139682-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal eGFP tag, inducible Blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB30
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(80..478)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 gttcctatac tttctagaga ataggaactt ccatatgtta gaaacaaatt tatttttaaa
       61 gttttatttt taataatttc tagccctccc acacataacc agagggcagc aattcacgaa
      121 tcccaactgc cgtcggctgt ccatcactgt ccttcactat ggctttgatc ccaggatgca
      181 gatcgagaag aacctgtcgg caccgtccgc aggggctcaa gatgcccctg ttctcatttc
      241 cgatcgcgac gatacaagtc aggttgccag ctgccgcagc agcagcagtg cccagcacca
      301 cgagttctgc gcaaggtccc ccagtaaaat gatatacatt gacaccagtg aagatgcggc
      361 cgtcgctaga gagagctgcg ctggcgacgc tgtagtcttc agagatgggg atgctgttga
      421 ttgtagccgt tgctctttca atgagggtgg attcttcttg agacaaaggc ttggccatat
      481 gcctaggctt gttgatttgt tttttgttta tttactgcgg taattcctag tcttagaagc
      541 ttattgaact tgttgatgcc ttctggttgg gaaatcctcc tctttatacc cgatccagtc
      601 caatcgaaac tgtgggccgt ggtgcccgat aagataagaa agccgtgtgc aagtcgatcg
      661 gcgtgcgcaa aagcataatg ctttttctgc ccggcgatga taagcgattt gcacacgccc
      721 tgataacagg aattgatttt tttggtgcgc aaagcagtga aagctctata agaatgctgg
      781 accgccagtc gacaatcatc aaacagtgct agctaacggc taagtgaatc aagtttatt
//
