LOCUS       sequence-139685-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template base vector for N-terminal tagging, constitutive 
            Blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB36
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(22..54)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             324..722
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 gctcgagttt ttcagcaaga taagttccta tactttctag agaataggaa cttccatatg
       61 ctgttggaat atactattca acctacaaaa ataacgttaa acaacactac tttatatttg
      121 atatgaatgg ccacaccttt tatgccataa aacatattgt aagagaatac cactcttttt
      181 attccttctt tccttcttgt acgttttttg ctgtgagtag gtcgtggtgc tggtgttgca
      241 gttgaaataa cttaaaatat aaatcataaa actcaaacat aaacttgact atttatttat
      301 ttattaagaa aggaggtacc ggtatggcca agcctttgtc tcaagaagaa tccaccctca
      361 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
      421 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
      481 ggggaccttg cgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
      541 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
      601 gccgacaggt tcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
      661 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
      721 agaaattatt aaaaataaaa ctttaaaaat aaatttgttt ctaacatatg cctaggcttg
      781 ttgatttgtt ttttgtttat ttactgcggt aattcctagt cttagaag
//
