LOCUS       sequence-139687-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template base vector for N-terminal tagging, constitutive 
            Blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB36
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    448..481
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tgtcaaaaga gacgtctttt gttaagaatg ctgaggaact tgcaaagcaa aaaatggatg
       61 ctattaaccc tgaactttct tcaaaattta aatttttaat aaaattcctg tctcagtttc
      121 ctgaagcttg ctctaaacct cgttcaaaaa aaatgcagaa taaagttggt caagaggaac
      181 atattgaata tttagctcgt agttttcatg agagtcgatt gccaagaaaa cccacgccac
      241 ctacaacggt tcctgatgag gtggttagca tagttcttaa tataagtttt aatatacagc
      301 ctgaaaatct tgagagaata aaagaagaac atcgattttc catggcagct gagaatattg
      361 taggagatct tctagaaaga tactagtacc gccgcttgga gcagctggag aggtaccgaa
      421 ttcaccggtg gatcccattt tggattagaa gttcctattc tctagaaagt ataggaactt
      481 ccctaggttt gtgttaaaat attttgttgt tgattatatt aaaataaact tgattcactt
      541 agccgttagc tagcactgtt tgatgattgt cgactggcgg tccagcattc ttatagagct
      601 ttcactgctt tgcgcaccaa aaaaatcaat tcctgttatc agggcgtgtg caaatcgctt
      661 atcatcgccg ggcagaaaaa gcattatgct tttgcgcacg ccgatcgact tgcacacggc
      721 tttcttatct tatcgggcac cacggcccac agtttcgatt ggactggatc gggtataaag
      781 aggaggattt cccaaccaga aggcatcaac aagttcaata agctt
//
