LOCUS       sequence-139691-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal 3x FLAG tag, constitutive Blasticidin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB34
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(16..48)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             318..716
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 gtttttcagc aagataagtt cctatacttt ctagagaata ggaacttcca tatgctgttg
       61 gaatatacta ttcaacctac aaaaataacg ttaaacaaca ctactttata tttgatatga
      121 atggccacac cttttatgcc ataaaacata ttgtaagaga ataccactct ttttattcct
      181 tctttccttc ttgtacgttt tttgctgtga gtaggtcgtg gtgctggtgt tgcagttgaa
      241 ataacttaaa atataaatca taaaactcaa acataaactt gactatttat ttatttatta
      301 agaaaggagg taccggtatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
      361 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
      421 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
      481 cttgcgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
      541 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
      601 aggttcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
      661 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctagaaat
      721 tattaaaaat aaaactttaa aaataaattt gtttctaaca tatgcctagg cttgttgatt
      781 tgttttttgt ttatttactg cggtaattcc tagtcttaga agcttattga acttgtt
//
